Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity. / Bengtström, Linda; Trier, Xenia; Granby, Kit; Rosenmai, Anna Kjerstine; Petersen, Jens Højslev.

In: Food Additives and Contaminants - Part A, Vol. 31, No. 7, 2014, p. 1291-1300.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bengtström, L, Trier, X, Granby, K, Rosenmai, AK & Petersen, JH 2014, 'Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity', Food Additives and Contaminants - Part A, vol. 31, no. 7, pp. 1291-1300. https://doi.org/10.1080/19440049.2014.912357

APA

Bengtström, L., Trier, X., Granby, K., Rosenmai, A. K., & Petersen, J. H. (2014). Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity. Food Additives and Contaminants - Part A, 31(7), 1291-1300. https://doi.org/10.1080/19440049.2014.912357

Vancouver

Bengtström L, Trier X, Granby K, Rosenmai AK, Petersen JH. Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity. Food Additives and Contaminants - Part A. 2014;31(7):1291-1300. https://doi.org/10.1080/19440049.2014.912357

Author

Bengtström, Linda ; Trier, Xenia ; Granby, Kit ; Rosenmai, Anna Kjerstine ; Petersen, Jens Højslev. / Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity. In: Food Additives and Contaminants - Part A. 2014 ; Vol. 31, No. 7. pp. 1291-1300.

Bibtex

@article{9e0a7a9632d942a78a17016221473533,
title = "Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity",
abstract = "Paper and board used as food contact materials (FCMs) are chemically complex matrices, partly due to the naturally occurring substances in paper and board, but also due to the chemical treatment of the paper used to make it suitable for food contact. In order to assure the safety of packaging materials, information on the exposure as well as on the toxicity of substances in the packaging must be obtained. This study describes a comprehensive method for the extraction and fractionation of substances present in paper and board FCMs for further investigation by in vitro testing and chemical analysis. The extraction efficiency and the fractionation process were validated by determining recoveries in extracts from paper and board fortified with five surrogates of known concentration. The recoveries for the five surrogates were between 20% and 104% in the raw extract and between 21% and 109% after extraction and fractionation. The fractionation both reduces the number of compounds to be identified and works as a sample clean-up by reducing matrix effects. Raw extracts and fractions from two paper and board FCMs were furthermore tested in the aryl hydrocarbon receptor (AhR) reporter gene assay. Both raw extracts and two of the fractions of the raw extracts gave a positive response in the AhR assay. The strategy of extraction followed by fractionation offers a powerful tool in order to make the workflow for screening FCMs for potentially adverse effects more efficient.",
keywords = "cardboard, extraction, food packaging, fractionation, in vitro toxicological screening, paper",
author = "Linda Bengtstr{\"o}m and Xenia Trier and Kit Granby and Rosenmai, {Anna Kjerstine} and Petersen, {Jens H{\o}jslev}",
year = "2014",
doi = "10.1080/19440049.2014.912357",
language = "English",
volume = "31",
pages = "1291--1300",
journal = "Food Additives and Contaminants",
issn = "0265-203X",
publisher = "Taylor & Francis",
number = "7",

}

RIS

TY - JOUR

T1 - Fractionation of extracts from paper and board food contact materials for in vitro screening of toxicity

AU - Bengtström, Linda

AU - Trier, Xenia

AU - Granby, Kit

AU - Rosenmai, Anna Kjerstine

AU - Petersen, Jens Højslev

PY - 2014

Y1 - 2014

N2 - Paper and board used as food contact materials (FCMs) are chemically complex matrices, partly due to the naturally occurring substances in paper and board, but also due to the chemical treatment of the paper used to make it suitable for food contact. In order to assure the safety of packaging materials, information on the exposure as well as on the toxicity of substances in the packaging must be obtained. This study describes a comprehensive method for the extraction and fractionation of substances present in paper and board FCMs for further investigation by in vitro testing and chemical analysis. The extraction efficiency and the fractionation process were validated by determining recoveries in extracts from paper and board fortified with five surrogates of known concentration. The recoveries for the five surrogates were between 20% and 104% in the raw extract and between 21% and 109% after extraction and fractionation. The fractionation both reduces the number of compounds to be identified and works as a sample clean-up by reducing matrix effects. Raw extracts and fractions from two paper and board FCMs were furthermore tested in the aryl hydrocarbon receptor (AhR) reporter gene assay. Both raw extracts and two of the fractions of the raw extracts gave a positive response in the AhR assay. The strategy of extraction followed by fractionation offers a powerful tool in order to make the workflow for screening FCMs for potentially adverse effects more efficient.

AB - Paper and board used as food contact materials (FCMs) are chemically complex matrices, partly due to the naturally occurring substances in paper and board, but also due to the chemical treatment of the paper used to make it suitable for food contact. In order to assure the safety of packaging materials, information on the exposure as well as on the toxicity of substances in the packaging must be obtained. This study describes a comprehensive method for the extraction and fractionation of substances present in paper and board FCMs for further investigation by in vitro testing and chemical analysis. The extraction efficiency and the fractionation process were validated by determining recoveries in extracts from paper and board fortified with five surrogates of known concentration. The recoveries for the five surrogates were between 20% and 104% in the raw extract and between 21% and 109% after extraction and fractionation. The fractionation both reduces the number of compounds to be identified and works as a sample clean-up by reducing matrix effects. Raw extracts and fractions from two paper and board FCMs were furthermore tested in the aryl hydrocarbon receptor (AhR) reporter gene assay. Both raw extracts and two of the fractions of the raw extracts gave a positive response in the AhR assay. The strategy of extraction followed by fractionation offers a powerful tool in order to make the workflow for screening FCMs for potentially adverse effects more efficient.

KW - cardboard

KW - extraction

KW - food packaging

KW - fractionation

KW - in vitro toxicological screening

KW - paper

U2 - 10.1080/19440049.2014.912357

DO - 10.1080/19440049.2014.912357

M3 - Journal article

C2 - 24816131

AN - SCOPUS:84902885908

VL - 31

SP - 1291

EP - 1300

JO - Food Additives and Contaminants

JF - Food Additives and Contaminants

SN - 0265-203X

IS - 7

ER -

ID: 333780548