First Evidence That Nematode Communities in Deadwood Are Related to Tree Species Identity and to Co-Occurring Fungi and Prokaryotes
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First Evidence That Nematode Communities in Deadwood Are Related to Tree Species Identity and to Co-Occurring Fungi and Prokaryotes. / Moll, Julia; Roy, Friederike; Bässler, Claus; Heilmann-Clausen, Jacob; Hofrichter, Martin; Kellner, Harald; Krabel, Doris; Schmidt, Jan Henrik; Buscot, François; Hoppe, Björn.
In: Microorganisms, Vol. 9, No. 7, 1454, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - First Evidence That Nematode Communities in Deadwood Are Related to Tree Species Identity and to Co-Occurring Fungi and Prokaryotes
AU - Moll, Julia
AU - Roy, Friederike
AU - Bässler, Claus
AU - Heilmann-Clausen, Jacob
AU - Hofrichter, Martin
AU - Kellner, Harald
AU - Krabel, Doris
AU - Schmidt, Jan Henrik
AU - Buscot, François
AU - Hoppe, Björn
N1 - Funding Information: Funding: This research was funded by Deutsche Forschungsgemeinschaft (DFG), grant numbers BA5127/1‐1, BU 941/26‐1, HO 1961/6‐1, HO 6077/1‐3 and KE 1742/2‐1. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Nematodes represent a diverse and ubiquitous group of metazoans in terrestrial environments. They feed on bacteria, fungi, plants, other nematodes or parasitize a variety of animals and hence may be considered as active members of many food webs. Deadwood is a structural component of forest ecosystems which harbors many niches for diverse biota. As fungi and bacteria are among the most prominent decomposing colonizers of deadwood, we anticipated frequent and diverse nematode populations to co‐occur in such ecosystems. However, knowledge about their ability to colonize this habitat is still limited. We applied DNA‐based amplicon sequencing (metabarcoding) of the 18S rRNA gene to analyze nematode communities in sapwood and heartwood of decaying logs from 13 different tree species. We identified 247 nematode ASVs (amplicon sequence variants) from 27 families. Most of these identified families represent bacterial and fungal feeders. Their composition strongly depended on tree species identity in both wood compartments. While pH and water content were the only wood properties that contributed to nematodes’ distribution, co‐occurring fungal and prokaryotic (bacteria and archaea) α‐ and β‐ diversities were significantly related to nematode communities. By exploring thirteen different tree species, which exhibit a broad range of wood characteristics, this study provides first and comprehensive insights into nematode diversity in deadwood of temperate forests and indicates connectivity to other wood‐inhabiting organisms.
AB - Nematodes represent a diverse and ubiquitous group of metazoans in terrestrial environments. They feed on bacteria, fungi, plants, other nematodes or parasitize a variety of animals and hence may be considered as active members of many food webs. Deadwood is a structural component of forest ecosystems which harbors many niches for diverse biota. As fungi and bacteria are among the most prominent decomposing colonizers of deadwood, we anticipated frequent and diverse nematode populations to co‐occur in such ecosystems. However, knowledge about their ability to colonize this habitat is still limited. We applied DNA‐based amplicon sequencing (metabarcoding) of the 18S rRNA gene to analyze nematode communities in sapwood and heartwood of decaying logs from 13 different tree species. We identified 247 nematode ASVs (amplicon sequence variants) from 27 families. Most of these identified families represent bacterial and fungal feeders. Their composition strongly depended on tree species identity in both wood compartments. While pH and water content were the only wood properties that contributed to nematodes’ distribution, co‐occurring fungal and prokaryotic (bacteria and archaea) α‐ and β‐ diversities were significantly related to nematode communities. By exploring thirteen different tree species, which exhibit a broad range of wood characteristics, this study provides first and comprehensive insights into nematode diversity in deadwood of temperate forests and indicates connectivity to other wood‐inhabiting organisms.
KW - Amplicon sequencing
KW - Bacteria
KW - Decomposition
KW - Metabarcoding
KW - Nematode diversity
KW - Temperate forest
KW - Trophic interactions
U2 - 10.3390/microorganisms9071454
DO - 10.3390/microorganisms9071454
M3 - Journal article
C2 - 34361890
AN - SCOPUS:85108991222
VL - 9
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 7
M1 - 1454
ER -
ID: 275831175