Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures. / Giudice, Rita Del; Paracini, Nicolò; Laursen, Tomas; Blanchet, Clement; Roosen-Runge, Felix; Cárdenas, Marité.

In: Molecules, Vol. 27, No. 21, 7628, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Giudice, RD, Paracini, N, Laursen, T, Blanchet, C, Roosen-Runge, F & Cárdenas, M 2022, 'Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures', Molecules, vol. 27, no. 21, 7628. https://doi.org/10.3390/molecules27217628

APA

Giudice, R. D., Paracini, N., Laursen, T., Blanchet, C., Roosen-Runge, F., & Cárdenas, M. (2022). Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures. Molecules, 27(21), [7628]. https://doi.org/10.3390/molecules27217628

Vancouver

Giudice RD, Paracini N, Laursen T, Blanchet C, Roosen-Runge F, Cárdenas M. Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures. Molecules. 2022;27(21). 7628. https://doi.org/10.3390/molecules27217628

Author

Giudice, Rita Del ; Paracini, Nicolò ; Laursen, Tomas ; Blanchet, Clement ; Roosen-Runge, Felix ; Cárdenas, Marité. / Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures. In: Molecules. 2022 ; Vol. 27, No. 21.

Bibtex

@article{2e9c044cc2554325bc457a4a53d6d171,

title = "Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures",

abstract = "Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.",

keywords = "bicelles, cryo-TEM, DLS, model membranes, SAXS",

author = "Giudice, {Rita Del} and Nicol{\`o} Paracini and Tomas Laursen and Clement Blanchet and Felix Roosen-Runge and Marit{\'e} C{\'a}rdenas",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

doi = "10.3390/molecules27217628",

language = "English",

volume = "27",

journal = "Molecules",

issn = "1420-3049",

publisher = "M D P I AG",

number = "21",

}

RIS

TY - JOUR

T1 - Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures

AU - Giudice, Rita Del

AU - Paracini, Nicolò

AU - Laursen, Tomas

AU - Blanchet, Clement

AU - Roosen-Runge, Felix

AU - Cárdenas, Marité

PY - 2022

Y1 - 2022

N2 - Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.

AB - Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.

KW - bicelles

KW - cryo-TEM

KW - DLS

KW - model membranes

KW - SAXS

U2 - 10.3390/molecules27217628

DO - 10.3390/molecules27217628

M3 - Journal article

C2 - 36364455

AN - SCOPUS:85141759223

VL - 27

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 21

M1 - 7628

ER -

ID: 327939703