Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle
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Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle. / Kristiansen, S; Hargreaves, Mark; Richter, Erik A.
In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 270, No. 1, 1996, p. E197-E201.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Exercise-induced increase in glucose transport, GLUT-4, and VAMP-2 in plasma membrane from human muscle
AU - Kristiansen, S
AU - Hargreaves, Mark
AU - Richter, Erik A.
PY - 1996
Y1 - 1996
N2 - A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.
AB - A major effect of muscle contractions is an increase in sarcolemmal glucose transport. We have used a recently developed technique to produce sarcolemmal giant vesicles from human muscle biopsy samples obtained before and after exercise. Six men exercised for 10 min at 50% maximal O2 uptake (Vo2max) and then to fatigue at 100% Vo2max (5.7 +/- 0.2 min). Vesicle glucose transport at 5 mM increased from 3.3 +/- 0.6 pmol.microgram-1.min-1 at rest to 6.6 +/- 1.0 pmol.microgram-1.min-1 at fatigue (mean +/- SE, n = 6, P < 0.05). This increase in glucose transport was associated with a 1.6-fold increase in vesicle GLUT-4 protein content. Glucose transport normalized to GLUT-4 protein content also increased with exercise, suggesting increased intrinsic activity of GLUT-4. Furthermore, exercise resulted in a 1.4-fold increase in sarcolemmal vesicle-associated membrane protein (VAMP-2) content, suggesting that muscle contractions may induce trafficking of GLUT-4-containing vesicles via a mechanism similar to neurotransmitter release. Our results demonstrate for the first time exercise-induced translocation of GLUT-4 and VAMP-2 to the plasma membrane of human muscle and increased sarcolemmal glucose transport.
KW - Adult
KW - Biological Transport
KW - Blotting, Western
KW - Cell Membrane
KW - Glucose
KW - Glucose Transporter Type 4
KW - Humans
KW - Male
KW - Membrane Proteins
KW - Monosaccharide Transport Proteins
KW - Muscle Proteins
KW - Muscles
KW - Physical Exertion
KW - R-SNARE Proteins
KW - Sarcolemma
M3 - Journal article
C2 - 8772493
VL - 270
SP - E197-E201
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1
ER -
ID: 154750965