Exercise training remodels inguinal white adipose tissue through adaptations in innervation, vascularization, and the extracellular matrix
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Exercise training remodels inguinal white adipose tissue through adaptations in innervation, vascularization, and the extracellular matrix. / Nigro, Pasquale; Vamvini, Maria; Yang, Jiekun; Caputo, Tiziana; Ho, Li Lun; Carbone, Nicholas P.; Papadopoulos, Danae; Conlin, Royce; He, Jie; Hirshman, Michael F.; White, Joseph D.; Robidoux, Jacques; Hickner, Robert C.; Nielsen, Søren; Pedersen, Bente K.; Kellis, Manolis; Middelbeek, Roeland J.W.; Goodyear, Laurie J.
In: Cell Reports, Vol. 42, No. 4, 112392, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Exercise training remodels inguinal white adipose tissue through adaptations in innervation, vascularization, and the extracellular matrix
AU - Nigro, Pasquale
AU - Vamvini, Maria
AU - Yang, Jiekun
AU - Caputo, Tiziana
AU - Ho, Li Lun
AU - Carbone, Nicholas P.
AU - Papadopoulos, Danae
AU - Conlin, Royce
AU - He, Jie
AU - Hirshman, Michael F.
AU - White, Joseph D.
AU - Robidoux, Jacques
AU - Hickner, Robert C.
AU - Nielsen, Søren
AU - Pedersen, Bente K.
AU - Kellis, Manolis
AU - Middelbeek, Roeland J.W.
AU - Goodyear, Laurie J.
N1 - Publisher Copyright: © 2023 The Authors
PY - 2023
Y1 - 2023
N2 - Inguinal white adipose tissue (iWAT) is essential for the beneficial effects of exercise training on metabolic health. The underlying mechanisms for these effects are not fully understood, and here, we test the hypothesis that exercise training results in a more favorable iWAT structural phenotype. Using biochemical, imaging, and multi-omics analyses, we find that 11 days of wheel running in male mice causes profound iWAT remodeling including decreased extracellular matrix (ECM) deposition and increased vascularization and innervation. We identify adipose stem cells as one of the main contributors to training-induced ECM remodeling, show that the PRDM16 transcriptional complex is necessary for iWAT remodeling and beiging, and discover neuronal growth regulator 1 (NEGR1) as a link between PRDM16 and neuritogenesis. Moreover, we find that training causes a shift from hypertrophic to insulin-sensitive adipocyte subpopulations. Exercise training leads to remarkable adaptations to iWAT structure and cell-type composition that can confer beneficial changes in tissue metabolism.
AB - Inguinal white adipose tissue (iWAT) is essential for the beneficial effects of exercise training on metabolic health. The underlying mechanisms for these effects are not fully understood, and here, we test the hypothesis that exercise training results in a more favorable iWAT structural phenotype. Using biochemical, imaging, and multi-omics analyses, we find that 11 days of wheel running in male mice causes profound iWAT remodeling including decreased extracellular matrix (ECM) deposition and increased vascularization and innervation. We identify adipose stem cells as one of the main contributors to training-induced ECM remodeling, show that the PRDM16 transcriptional complex is necessary for iWAT remodeling and beiging, and discover neuronal growth regulator 1 (NEGR1) as a link between PRDM16 and neuritogenesis. Moreover, we find that training causes a shift from hypertrophic to insulin-sensitive adipocyte subpopulations. Exercise training leads to remarkable adaptations to iWAT structure and cell-type composition that can confer beneficial changes in tissue metabolism.
KW - Adipo-Clear
KW - CP: Metabolism
KW - CP: Molecular biology
KW - ECM
KW - exercise
KW - innervation
KW - NEGR1
KW - PRDM16
KW - proteomics
KW - spatial transcriptomics
KW - vascularization
KW - white adipose tissue
U2 - 10.1016/j.celrep.2023.112392
DO - 10.1016/j.celrep.2023.112392
M3 - Journal article
C2 - 37058410
AN - SCOPUS:85152296501
VL - 42
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 4
M1 - 112392
ER -
ID: 362891079