EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression

Research output: Contribution to journal › Journal article › Research › peer-review

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EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. / Kalisz, Mark; Winzi, Maria Karin; Bisgaard, Hanne Cathrine; Serup, Palle.

In: Developmental Biology, Vol. 362, No. 1, 01.02.2012, p. 94-103.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kalisz, M, Winzi, MK, Bisgaard, HC & Serup, P 2012, 'EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression', Developmental Biology, vol. 362, no. 1, pp. 94-103. https://doi.org/10.1016/j.ydbio.2011.11.017

APA

Kalisz, M., Winzi, M. K., Bisgaard, H. C., & Serup, P. (2012). EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. Developmental Biology, 362(1), 94-103. https://doi.org/10.1016/j.ydbio.2011.11.017

Vancouver

Kalisz M, Winzi MK, Bisgaard HC, Serup P. EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. Developmental Biology. 2012 Feb 1;362(1):94-103. https://doi.org/10.1016/j.ydbio.2011.11.017

Author

Kalisz, Mark ; Winzi, Maria Karin ; Bisgaard, Hanne Cathrine ; Serup, Palle. / EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression. In: Developmental Biology. 2012 ; Vol. 362, No. 1. pp. 94-103.

Bibtex

@article{79f37a8b8d4f4e4498aeb84030ba3676,

title = "EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression",

abstract = "TGF{\ss} signaling patterns the primitive streak, yet little is known about transcriptional effectors that mediate the cell fate choices during streak-like development in mammalian embryos and in embryonic stem (ES) cells. Here we demonstrate that cross-antagonistic actions of EVEN-SKIPPED HOMEOBOX 1 (EVX1) and GOOSECOID (GSC) regulate cell fate decisions in streak-like progenitors derived from human ES cells exposed to BMP4 and/or activin. We found that EVX1 repressed GSC expression and promoted formation of posterior streak-like progeny in response to BMP4, and conversely that GSC repressed EVX1 expression and was required for development of anterior streak-like progeny in response to activin. Chromatin immunoprecipitation assays showed that EVX1 bound to the GSC 5'-flanking region in BMP4 treated human ES cells, and band shift assays identified two EVX1 binding sites in the GSC 5'-region. Significantly, we found that intact EVX1 binding sites were required for BMP4-mediated repression of GSC reporter constructs. We conclude that BMP4-induced EVX1 repress GSC directly and the two genes form the core of a gene regulatory network (GRN) controlling cell fates in streak-like human ES cell progeny.",

author = "Mark Kalisz and Winzi, {Maria Karin} and Bisgaard, {Hanne Cathrine} and Palle Serup",

year = "2012",

month = feb,

day = "1",

doi = "10.1016/j.ydbio.2011.11.017",

language = "English",

volume = "362",

pages = "94--103",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Academic Press",

number = "1",

}

RIS

TY - JOUR

T1 - EVEN-SKIPPED HOMEOBOX 1 controls human ES cell differentiation by directly repressing GOOSECOID expression

AU - Kalisz, Mark

AU - Winzi, Maria Karin

AU - Bisgaard, Hanne Cathrine

AU - Serup, Palle

PY - 2012/2/1

Y1 - 2012/2/1

N2 - TGFß signaling patterns the primitive streak, yet little is known about transcriptional effectors that mediate the cell fate choices during streak-like development in mammalian embryos and in embryonic stem (ES) cells. Here we demonstrate that cross-antagonistic actions of EVEN-SKIPPED HOMEOBOX 1 (EVX1) and GOOSECOID (GSC) regulate cell fate decisions in streak-like progenitors derived from human ES cells exposed to BMP4 and/or activin. We found that EVX1 repressed GSC expression and promoted formation of posterior streak-like progeny in response to BMP4, and conversely that GSC repressed EVX1 expression and was required for development of anterior streak-like progeny in response to activin. Chromatin immunoprecipitation assays showed that EVX1 bound to the GSC 5'-flanking region in BMP4 treated human ES cells, and band shift assays identified two EVX1 binding sites in the GSC 5'-region. Significantly, we found that intact EVX1 binding sites were required for BMP4-mediated repression of GSC reporter constructs. We conclude that BMP4-induced EVX1 repress GSC directly and the two genes form the core of a gene regulatory network (GRN) controlling cell fates in streak-like human ES cell progeny.

AB - TGFß signaling patterns the primitive streak, yet little is known about transcriptional effectors that mediate the cell fate choices during streak-like development in mammalian embryos and in embryonic stem (ES) cells. Here we demonstrate that cross-antagonistic actions of EVEN-SKIPPED HOMEOBOX 1 (EVX1) and GOOSECOID (GSC) regulate cell fate decisions in streak-like progenitors derived from human ES cells exposed to BMP4 and/or activin. We found that EVX1 repressed GSC expression and promoted formation of posterior streak-like progeny in response to BMP4, and conversely that GSC repressed EVX1 expression and was required for development of anterior streak-like progeny in response to activin. Chromatin immunoprecipitation assays showed that EVX1 bound to the GSC 5'-flanking region in BMP4 treated human ES cells, and band shift assays identified two EVX1 binding sites in the GSC 5'-region. Significantly, we found that intact EVX1 binding sites were required for BMP4-mediated repression of GSC reporter constructs. We conclude that BMP4-induced EVX1 repress GSC directly and the two genes form the core of a gene regulatory network (GRN) controlling cell fates in streak-like human ES cell progeny.

U2 - 10.1016/j.ydbio.2011.11.017

DO - 10.1016/j.ydbio.2011.11.017

M3 - Journal article

C2 - 22178155

VL - 362

SP - 94

EP - 103

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -

ID: 36074021