Engineering of Salmonella Phages into Novel Antimicrobial Tailocins
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Engineering of Salmonella Phages into Novel Antimicrobial Tailocins. / Woudstra, Cedric; Sørensen, Anders Nørgaard; Brøndsted, Lone.
In: Cells, Vol. 12, No. 22, 2637, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Engineering of Salmonella Phages into Novel Antimicrobial Tailocins
AU - Woudstra, Cedric
AU - Sørensen, Anders Nørgaard
AU - Brøndsted, Lone
PY - 2023
Y1 - 2023
N2 - Due to the extensive use of antibiotics, the increase of infections caused by antibiotic-resistant bacteria is now a global health concern. Phages have proven useful for treating bacterial infections and represent a promising alternative or complement to antibiotic treatment. Yet, other alternatives exist, such as bacteria-produced non-replicative protein complexes that can kill their targeted bacteria by puncturing their membrane (Tailocins). To expand the repertoire of Tailocins available, we suggest a new approach that transforms phages into Tailocins. Here, we genetically engineered the virulent Ackermannviridae phage S117, as well as temperate phages Fels-1, -2 and Gifsy-1 and -2, targeting the food pathogen Salmonella, by deleting the portal vertex or major capsid gene using CRISPR-Cas9. We report the production of Tailocin particles from engineered virulent and temperate phages able to kill their native host. Our work represents a steppingstone that taps into the huge diversity of phages and transforms them into versatile puncturing new antimicrobials.
AB - Due to the extensive use of antibiotics, the increase of infections caused by antibiotic-resistant bacteria is now a global health concern. Phages have proven useful for treating bacterial infections and represent a promising alternative or complement to antibiotic treatment. Yet, other alternatives exist, such as bacteria-produced non-replicative protein complexes that can kill their targeted bacteria by puncturing their membrane (Tailocins). To expand the repertoire of Tailocins available, we suggest a new approach that transforms phages into Tailocins. Here, we genetically engineered the virulent Ackermannviridae phage S117, as well as temperate phages Fels-1, -2 and Gifsy-1 and -2, targeting the food pathogen Salmonella, by deleting the portal vertex or major capsid gene using CRISPR-Cas9. We report the production of Tailocin particles from engineered virulent and temperate phages able to kill their native host. Our work represents a steppingstone that taps into the huge diversity of phages and transforms them into versatile puncturing new antimicrobials.
KW - Salmonella Phages/genetics
KW - Bacteriophages/genetics
KW - Anti-Infective Agents
KW - Anti-Bacterial Agents/pharmacology
KW - Salmonella
KW - Bacteria
U2 - 10.3390/cells12222637
DO - 10.3390/cells12222637
M3 - Journal article
C2 - 37998371
VL - 12
JO - Cells
JF - Cells
SN - 2073-4409
IS - 22
M1 - 2637
ER -
ID: 374523777