Effects of rituximab and dexamethasone on regulatory and proinflammatory B-cell subsets in patients with primary immune thrombocytopenia
Research output: Contribution to journal › Journal article › Research › peer-review
OBJECTIVE: To investigate the cytokine production and surface marker composition of B cells in adult patients with newly diagnosed primary immune thrombocytopenia (ITP) before and 12 months after treatment with rituximab + dexamethasone (RTX+DXM) or dexamethasone (DXM).
METHODS: Peripheral blood mononuclear cells were isolated from nine patients treated with RTX+DXM, seven patients treated with DXM, and seven healthy donors. Expression of the cell-surface markers CD5, CD27, CD25, and CD19, and intracellular content of IL-6 and IL-10 were measured by flow cytometry.
RESULTS: PBMCs from ITP patients at baseline contained a lower proportion of IL-10+ B cells (P < .01) and IL-6+ B cells (P < .01) than healthy controls. All patients responded to therapy and levels were normalized at 12 months. The proportion of CD5+ B cells increased (P < .01) and CD27+ memory B cells decreased (P < .05) 12 months after treatment with RTX+DXM compared to baseline, with an inverse correlation between platelet numbers and the proportion of CD27+ B cells (R = -0.71; P < .05).
CONCLUSION: Both treatment regimens normalized the frequencies of cytokine-producing B cells. The additional increase in CD5+ B cells after RTX+DXM is compatible with induction of Bregs.
Original language | English |
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Journal | European Journal of Haematology |
Volume | 100 |
Issue number | 1 |
Pages (from-to) | 45-52 |
ISSN | 0902-4441 |
DOIs | |
Publication status | Published - 2018 |
- Adult, Aged, Aged, 80 and over, Autoantibodies/blood, B-Lymphocyte Subsets/drug effects, B-Lymphocytes, Regulatory/drug effects, Biomarkers, Case-Control Studies, Cytokines/metabolism, Dexamethasone/pharmacology, Female, Humans, Immunologic Factors/pharmacology, Immunophenotyping, Lymphocyte Depletion, Male, Middle Aged, Phenotype, Platelet Count, Purpura, Thrombocytopenic, Idiopathic/diagnosis, Rituximab/pharmacology, Young Adult
Research areas
ID: 222169225