E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. / Porse, B T; Pedersen TA; Xu, X; Lindberg, B; Wewer, U M; Friis-Hansen, L; Nerlov, C.

In: Cell, Vol. 107, No. 2, 2001, p. 247-58.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Porse, BT, Pedersen TA, Xu, X, Lindberg, B, Wewer, UM, Friis-Hansen, L & Nerlov, C 2001, 'E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo.', Cell, vol. 107, no. 2, pp. 247-58.

APA

Porse, B. T., Pedersen TA, Xu, X., Lindberg, B., Wewer, U. M., Friis-Hansen, L., & Nerlov, C. (2001). E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. Cell, 107(2), 247-58.

Vancouver

Porse BT, Pedersen TA, Xu X, Lindberg B, Wewer UM, Friis-Hansen L et al. E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. Cell. 2001;107(2):247-58.

Author

Porse, B T ; Pedersen TA ; Xu, X ; Lindberg, B ; Wewer, U M ; Friis-Hansen, L ; Nerlov, C. / E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo. In: Cell. 2001 ; Vol. 107, No. 2. pp. 247-58.

Bibtex

@article{a838adb0588e11dd8d9f000ea68e967b,
title = "E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo.",
abstract = "The C/EBPalpha transcription factor is required for differentiation of adipocytes and neutrophil granulocytes, and controls cellular proliferation in vivo. To address the molecular mechanisms of C/EBPalpha action, we have identified C/EBPalpha mutants defective in repression of E2F-dependent transcription and found them to be impaired in their ability to suppress cellular proliferation, and to induce adipocyte differentiation in vitro. Using targeted mutagenesis of the mouse germline, we show that E2F repression-deficient C/EBPalpha alleles failed to support adipocyte and granulocyte differentiation in vivo. These results indicate that E2F repression by C/EBPalpha is critical for its ability to induce terminal differentiation, and thus provide genetic evidence that direct cell cycle control by a mammalian lineage-instructive transcription factor couples cellular growth arrest and differentiation.",
author = "Porse, {B T} and {Pedersen TA} and X Xu and B Lindberg and Wewer, {U M} and L Friis-Hansen and C Nerlov",
note = "Keywords: 3T3 Cells; Adipocytes; Alleles; Amino Acid Sequence; Animals; Blotting, Northern; Blotting, Western; CCAAT-Enhancer-Binding Protein-alpha; Cell Cycle Proteins; Cell Differentiation; Cell Division; DNA-Binding Proteins; E2F Transcription Factors; Female; Flow Cytometry; Genes, Reporter; Glutathione Transferase; Granulocytes; Humans; Mice; Mice, Knockout; Mice, Transgenic; Molecular Sequence Data; Mutagenesis, Site-Directed; Ovary; Protein Binding; Rats; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors; Transcription, Genetic",
year = "2001",
language = "English",
volume = "107",
pages = "247--58",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - E2F repression by C/EBPalpha is required for adipogenesis and granulopoiesis in vivo.

AU - Porse, B T

AU - Pedersen TA, null

AU - Xu, X

AU - Lindberg, B

AU - Wewer, U M

AU - Friis-Hansen, L

AU - Nerlov, C

N1 - Keywords: 3T3 Cells; Adipocytes; Alleles; Amino Acid Sequence; Animals; Blotting, Northern; Blotting, Western; CCAAT-Enhancer-Binding Protein-alpha; Cell Cycle Proteins; Cell Differentiation; Cell Division; DNA-Binding Proteins; E2F Transcription Factors; Female; Flow Cytometry; Genes, Reporter; Glutathione Transferase; Granulocytes; Humans; Mice; Mice, Knockout; Mice, Transgenic; Molecular Sequence Data; Mutagenesis, Site-Directed; Ovary; Protein Binding; Rats; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors; Transcription, Genetic

PY - 2001

Y1 - 2001

N2 - The C/EBPalpha transcription factor is required for differentiation of adipocytes and neutrophil granulocytes, and controls cellular proliferation in vivo. To address the molecular mechanisms of C/EBPalpha action, we have identified C/EBPalpha mutants defective in repression of E2F-dependent transcription and found them to be impaired in their ability to suppress cellular proliferation, and to induce adipocyte differentiation in vitro. Using targeted mutagenesis of the mouse germline, we show that E2F repression-deficient C/EBPalpha alleles failed to support adipocyte and granulocyte differentiation in vivo. These results indicate that E2F repression by C/EBPalpha is critical for its ability to induce terminal differentiation, and thus provide genetic evidence that direct cell cycle control by a mammalian lineage-instructive transcription factor couples cellular growth arrest and differentiation.

AB - The C/EBPalpha transcription factor is required for differentiation of adipocytes and neutrophil granulocytes, and controls cellular proliferation in vivo. To address the molecular mechanisms of C/EBPalpha action, we have identified C/EBPalpha mutants defective in repression of E2F-dependent transcription and found them to be impaired in their ability to suppress cellular proliferation, and to induce adipocyte differentiation in vitro. Using targeted mutagenesis of the mouse germline, we show that E2F repression-deficient C/EBPalpha alleles failed to support adipocyte and granulocyte differentiation in vivo. These results indicate that E2F repression by C/EBPalpha is critical for its ability to induce terminal differentiation, and thus provide genetic evidence that direct cell cycle control by a mammalian lineage-instructive transcription factor couples cellular growth arrest and differentiation.

M3 - Journal article

C2 - 11672531

VL - 107

SP - 247

EP - 258

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -

ID: 5140683