Drug delivery by phospholipase A2 degradable liposomes
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Drug delivery by phospholipase A2 degradable liposomes. / Davidsen, Jesper; Vermehren, Charlotte; Frokjaer, Sven; Mouritsen, Ole G.; Jørgensen, Kent.
In: International Journal of Pharmaceutics, Vol. 214, No. 1-2, 19.02.2001, p. 67-69.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Drug delivery by phospholipase A2 degradable liposomes
AU - Davidsen, Jesper
AU - Vermehren, Charlotte
AU - Frokjaer, Sven
AU - Mouritsen, Ole G.
AU - Jørgensen, Kent
PY - 2001/2/19
Y1 - 2001/2/19
N2 - The effect of poly(ethylene glycol)-phospholipid (PE-PEG) lipopolymers on phospholipase A2 (PLA2) hydrolysis of liposomes composed of stearoyl-oleoylphosphatidylcholine (SOPC) was investigated. The PLA2 lag-time, which is inversely related to the enzymatic activity, was determined by fluorescence, and the zeta-potentials of the liposomes were measured as a function of PE-PEG lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzymatic activity, was observed with increasing amounts of the negatively charged PE-PEG lipopolymers incorporated into the SOPC liposomes. The enhancement of the PLA2 enzymatic activity might involve a stronger PLA2 binding affinity towards the negatively charged and polymer covered PEG liposomes.
AB - The effect of poly(ethylene glycol)-phospholipid (PE-PEG) lipopolymers on phospholipase A2 (PLA2) hydrolysis of liposomes composed of stearoyl-oleoylphosphatidylcholine (SOPC) was investigated. The PLA2 lag-time, which is inversely related to the enzymatic activity, was determined by fluorescence, and the zeta-potentials of the liposomes were measured as a function of PE-PEG lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzymatic activity, was observed with increasing amounts of the negatively charged PE-PEG lipopolymers incorporated into the SOPC liposomes. The enhancement of the PLA2 enzymatic activity might involve a stronger PLA2 binding affinity towards the negatively charged and polymer covered PEG liposomes.
KW - Degradation
KW - Drug-delivery
KW - Lipopolymer
KW - Liposomes
KW - PEG liposomes
KW - Phospholipase A
UR - http://www.scopus.com/inward/record.url?scp=0035910868&partnerID=8YFLogxK
U2 - 10.1016/S0378-5173(00)00634-7
DO - 10.1016/S0378-5173(00)00634-7
M3 - Journal article
C2 - 11282239
AN - SCOPUS:0035910868
VL - 214
SP - 67
EP - 69
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 1-2
ER -
ID: 230987712