Directly from the source: endogenous preparations of molecular machines
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Directly from the source : endogenous preparations of molecular machines. / Mesa, Pablo; Deniaud, Aurélien; Montoya, Guillermo; Schaffitzel, Christiane.
In: Current Opinion in Structural Biology, Vol. 23, No. 3, 06.2013, p. 319-25.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Directly from the source
T2 - endogenous preparations of molecular machines
AU - Mesa, Pablo
AU - Deniaud, Aurélien
AU - Montoya, Guillermo
AU - Schaffitzel, Christiane
N1 - Copyright © 2013. Published by Elsevier Ltd.
PY - 2013/6
Y1 - 2013/6
N2 - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.
AB - Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.
KW - Analytic Sample Preparation Methods
KW - Animals
KW - Bacteria/chemistry
KW - Crystallization
KW - DNA, Recombinant/genetics
KW - Databases, Protein
KW - Humans
KW - Multiprotein Complexes/chemistry
KW - Protein Conformation
KW - Recombinant Proteins/biosynthesis
KW - Yeasts/chemistry
U2 - 10.1016/j.sbi.2013.01.005
DO - 10.1016/j.sbi.2013.01.005
M3 - Review
C2 - 23395510
VL - 23
SP - 319
EP - 325
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
SN - 0959-440X
IS - 3
ER -
ID: 246724916