Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis
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Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis. / Martel, Britta Cathrina; Dyring-Andersen, Beatrice; Skov, Lone; Thestrup-Pedersen, Kristian; Skov, Søren; Skak, Kresten; Poulsen, Lars K.
In: Inflammation Research, Vol. 65, No. 4, 04.2016, p. 265-72.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis
AU - Martel, Britta Cathrina
AU - Dyring-Andersen, Beatrice
AU - Skov, Lone
AU - Thestrup-Pedersen, Kristian
AU - Skov, Søren
AU - Skak, Kresten
AU - Poulsen, Lars K
PY - 2016/4
Y1 - 2016/4
N2 - OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.
AB - OBJECTIVES: To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL: Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS: Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS: A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION: The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1007/s00011-015-0912-z
DO - 10.1007/s00011-015-0912-z
M3 - Journal article
C2 - 26722669
VL - 65
SP - 265
EP - 272
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 4
ER -
ID: 165663828