Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains
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Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains. / Holmfeldt, Karin; Odic, Dusko; Sullivan, Matthew B.; Middelboe, Mathias; Riemann, Lasse.
In: Applied and Environmental Microbiology, Vol. 78, No. 3, 2012, p. 892-894.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cultivated single stranded DNA phages that infect marine Bacteroidetes prove difficult to detect with DNA binding stains
AU - Holmfeldt, Karin
AU - Odic, Dusko
AU - Sullivan, Matthew B.
AU - Middelboe, Mathias
AU - Riemann, Lasse
PY - 2012
Y1 - 2012
N2 - This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.
AB - This is the first description of cultivated icosahedral single stranded DNA (ssDNA) phages isolated on heterotrophic marine bacterioplankton and with Bacteroidetes hosts. None of the 8 phages stained well with DNA binding stains, suggesting that in situ abundances of ssDNA phages are drastically underestimated using conventional methods for enumeration.
U2 - 10.1128/AEM.06580-11
DO - 10.1128/AEM.06580-11
M3 - Journal article
VL - 78
SP - 892
EP - 894
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 3
ER -
ID: 35320439