Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality

Research output: Contribution to journal › Journal article › Research › peer-review

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Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality. / Zaghet, Nico; Madsen, Katrine; Rossi, Federico; Perez, Daniel Fernandez; Amendola, Pier Giorgio; Demharter, Samuel; Pfisterer, Ulrich; Khodosevich, Konstantin; Pasini, Diego; Salcini, Anna Elisabetta.

In: Cell Reports, Vol. 37, No. 8, 2021, p. 110050.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zaghet, N, Madsen, K, Rossi, F, Perez, DF, Amendola, PG, Demharter, S, Pfisterer, U, Khodosevich, K, Pasini, D & Salcini, AE 2021, 'Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality', Cell Reports, vol. 37, no. 8, pp. 110050. https://doi.org/10.1016/j.celrep.2021.110050

APA

Zaghet, N., Madsen, K., Rossi, F., Perez, D. F., Amendola, P. G., Demharter, S., Pfisterer, U., Khodosevich, K., Pasini, D., & Salcini, A. E. (2021). Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality. Cell Reports, 37(8), 110050. https://doi.org/10.1016/j.celrep.2021.110050

Vancouver

Zaghet N, Madsen K, Rossi F, Perez DF, Amendola PG, Demharter S et al. Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality. Cell Reports. 2021;37(8):110050. https://doi.org/10.1016/j.celrep.2021.110050

Author

Zaghet, Nico ; Madsen, Katrine ; Rossi, Federico ; Perez, Daniel Fernandez ; Amendola, Pier Giorgio ; Demharter, Samuel ; Pfisterer, Ulrich ; Khodosevich, Konstantin ; Pasini, Diego ; Salcini, Anna Elisabetta. / Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality. In: Cell Reports. 2021 ; Vol. 37, No. 8. pp. 110050.

Bibtex

@article{eaf0bf7ee30f4a4e8f397f03266e68b8,

title = "Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality",

abstract = "Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.",

author = "Nico Zaghet and Katrine Madsen and Federico Rossi and Perez, {Daniel Fernandez} and Amendola, {Pier Giorgio} and Samuel Demharter and Ulrich Pfisterer and Konstantin Khodosevich and Diego Pasini and Salcini, {Anna Elisabetta}",

year = "2021",

doi = "10.1016/j.celrep.2021.110050",

language = "English",

volume = "37",

pages = "110050",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "8",

}

RIS

TY - JOUR

T1 - Coordinated maintenance of H3K36/K27 methylation by histone demethylases preserves germ cell identity and immortality

AU - Zaghet, Nico

AU - Madsen, Katrine

AU - Rossi, Federico

AU - Perez, Daniel Fernandez

AU - Amendola, Pier Giorgio

AU - Demharter, Samuel

AU - Pfisterer, Ulrich

AU - Khodosevich, Konstantin

AU - Pasini, Diego

AU - Salcini, Anna Elisabetta

PY - 2021

Y1 - 2021

N2 - Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.

AB - Germ cells have evolved unique mechanisms to ensure the transmission of genetically and nongenetically encoded information, whose alteration compromises germ cell immortality. Chromatin factors play fundamental roles in these mechanisms. H3K36 and H3K27 methyltransferases shape and propagate a pattern of histone methylation essential for C. elegans germ cell maintenance, but the role of respective histone demethylases remains unexplored. Here, we show that jmjd-5 regulates H3K36me2 and H3K27me3 levels, preserves germline immortality, and protects germ cell identity by controlling gene expression. The transcriptional and biological effects of jmjd-5 loss can be hindered by the removal of H3K27demethylases, indicating that H3K36/K27 demethylases act in a transcriptional framework and promote the balance between H3K36 and H3K27 methylation required for germ cell immortality. Furthermore, we find that in wild-type, but not in jmjd-5 mutants, alterations of H3K36 methylation and transcription occur at high temperature, suggesting a role for jmjd-5 in adaptation to environmental changes.

U2 - 10.1016/j.celrep.2021.110050

DO - 10.1016/j.celrep.2021.110050

M3 - Journal article

C2 - 34818537

VL - 37

SP - 110050

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 8

ER -

ID: 285883211