Complement component 3 and its activation split-products in saliva associate with periodontitis
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Complement component 3 and its activation split-products in saliva associate with periodontitis. / Damgaard, Christian; Massarenti, Laura; Danielsen, Anne Katrine; Graversen, Jonas H.; Holmstrup, Palle; Nielsen, Claus H.; Palarasah, Yaseelan.
In: Journal of Periodontology, Vol. 93, No. 9, 2022, p. 1294-1301.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Complement component 3 and its activation split-products in saliva associate with periodontitis
AU - Damgaard, Christian
AU - Massarenti, Laura
AU - Danielsen, Anne Katrine
AU - Graversen, Jonas H.
AU - Holmstrup, Palle
AU - Nielsen, Claus H.
AU - Palarasah, Yaseelan
N1 - Publisher Copyright: © 2022 American Academy of Periodontology.
PY - 2022
Y1 - 2022
N2 - Background: Periodontitis (PD) is classified by Grades A through C according to the risk of further progression, PD Grade C (PD-C) being the most severe progressing form. It is a matter of controversy, whether the disease activity observed in PD-C is due to impaired immune reactivity toward bacteria embedded in biofilms or a hyper-reactive immune response causing tissue damage as a bystander phenomenon. Little is known about the role of complement in this respect. Methods: Plasma and unstimulated saliva samples were collected from patients with PD-B (n = 34) or -C (n = 27) and healthy controls (HCs) (n = 28). Salivary and plasma levels of total C3, C3c, and C3dg were quantified using sandwich enzyme-linked immunosorbent assay (ELISA). Results: Salivary levels of total C3 and C3dg were elevated in PD-B and PD-C patients compared to HCs (both P < 0.05), while the levels of C3c were elevated in PD-C compared to HCs. Plasma levels of C3c were higher in PD-B patients than in HCs (P < 0.05). Conclusion: PD-B and PD-C patients show increased complement activation compared to HCs, but no difference was found between the two disease grades. PD-B, but not PD-C, is associated with increased systemic complement activation as assessed by C3c in plasma.
AB - Background: Periodontitis (PD) is classified by Grades A through C according to the risk of further progression, PD Grade C (PD-C) being the most severe progressing form. It is a matter of controversy, whether the disease activity observed in PD-C is due to impaired immune reactivity toward bacteria embedded in biofilms or a hyper-reactive immune response causing tissue damage as a bystander phenomenon. Little is known about the role of complement in this respect. Methods: Plasma and unstimulated saliva samples were collected from patients with PD-B (n = 34) or -C (n = 27) and healthy controls (HCs) (n = 28). Salivary and plasma levels of total C3, C3c, and C3dg were quantified using sandwich enzyme-linked immunosorbent assay (ELISA). Results: Salivary levels of total C3 and C3dg were elevated in PD-B and PD-C patients compared to HCs (both P < 0.05), while the levels of C3c were elevated in PD-C compared to HCs. Plasma levels of C3c were higher in PD-B patients than in HCs (P < 0.05). Conclusion: PD-B and PD-C patients show increased complement activation compared to HCs, but no difference was found between the two disease grades. PD-B, but not PD-C, is associated with increased systemic complement activation as assessed by C3c in plasma.
KW - complement component 3
KW - immunology
KW - inflammation and innate immunity
KW - pathogenesis
KW - periodontitis
KW - saliva
U2 - 10.1002/JPER.21-0530
DO - 10.1002/JPER.21-0530
M3 - Journal article
C2 - 35218227
AN - SCOPUS:85127379355
VL - 93
SP - 1294
EP - 1301
JO - Journal of Periodontology
JF - Journal of Periodontology
SN - 0022-3492
IS - 9
ER -
ID: 303682978