Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection
Research output: Contribution to journal › Journal article › Research › peer-review
A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.
Original language | English |
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Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
Volume | 713 |
Issue number | 2 |
Pages (from-to) | 379-386 |
Number of pages | 8 |
ISSN | 1572-6495 |
DOIs | |
Publication status | Published - 1998 |
- Acacetin, Apigenin
Research areas
ID: 254771801