Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection
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Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection. / Nielsen, Salka E; Dragsted, Lars Ove.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 713, No. 2, 1998, p. 379-386.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Column-switching high-performance liquid chromatographic assay for determination of apigenin and acacetin in human urine with ultraviolet absorbance detection
AU - Nielsen, Salka E
AU - Dragsted, Lars Ove
N1 - (Ekstern)
PY - 1998
Y1 - 1998
N2 - A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.
AB - A high-performance liquid chromatographic (HPLC) method is described for the determination of apigenin and the 4'-methylated derivative acacetin in human urine using column-switching and ultraviolet (UV) absorbance detection. Urine samples were enzymatically hydrolysed and solid-phase extracted prior to injection onto the HPLC system. Prior to elution of apigenin and the internal standard, 5,7,8-trihydroxyflavone, from the first column used for sample clean-up, the six-port valve was switched to the second column for analysis with UV detection. Detection of apigenin was precise and reproducible, with a limit of quantification of 10 ngml-1 urine. Detection and quantification of acacetin was linear down to 70 ngml-1 urine. The method has been successfully applied to determine the level of apigenin in 100 human urine samples from an intervention study with parsley. Copyright (C) 1998 Elsevier Science B.V.
KW - Acacetin
KW - Apigenin
UR - http://www.scopus.com/inward/record.url?scp=0032424649&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(98)00187-X
DO - 10.1016/S0378-4347(98)00187-X
M3 - Journal article
C2 - 9746253
AN - SCOPUS:0032424649
VL - 713
SP - 379
EP - 386
JO - Journal of Chromatography - Biomedical Applications
JF - Journal of Chromatography - Biomedical Applications
SN - 0378-4347
IS - 2
ER -
ID: 254771801