Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region.
Research output: Contribution to journal › Journal article › Research › peer-review
We have isolated four lambda-phages covering the complete pig aminopeptidase N/CD13 gene. The sequence of 2.85 kbp encompasses 1.18 kbp of the 5' upstream region and 1.67 kbp of the structural gene. In the promoter region we find a TATA box and potential binding sites for CTF-1/NF-1 and AP-2. By sequence comparisons we have found three domains showing similarity to promoter regions of the genes encoding human alpha 1-antitrypsin and human intestinal alkaline phosphatase. The gene sequence includes the first three exons and two introns. It shows that a single exon encodes the cytoplasmic tail, the membrane anchor and the junctional peptide.
| Original language | English |
|---|---|
| Journal | FEBS Letters |
| Volume | 251 |
| Issue number | 1-2 |
| Pages (from-to) | 275-81 |
| Number of pages | 6 |
| ISSN | 0014-5793 |
| Publication status | Published - 1989 |
Bibliographical note
Keywords: Alkaline Phosphatase; Amino Acid Sequence; Aminopeptidases; Animals; Antigens, CD; Antigens, CD13; Antigens, Differentiation, Myelomonocytic; Aspergillus Nuclease S1; Bacteriophage lambda; Base Sequence; Cell Membrane; Cloning, Molecular; DNA; DNA Restriction Enzymes; Endonucleases; Exons; Humans; Introns; Molecular Sequence Data; Nucleic Acid Hybridization; Promoter Regions (Genetics); RNA, Messenger; Regulatory Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid; Swine; Transcription, Genetic; alpha 1-Antitrypsin
ID: 6586522