Chemokine Oligomers and the Impact of Fondaparinux Binding
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Heparin, a widely used clinical anticoagulant, is generally well-tolerated; however, approximately 1% of patients develop heparin-induced thrombocytopenia (HIT), a serious side effect. While efforts to understand the role of chemokines in HIT development are ongoing, certain aspects remain less studied, such as the stabilization of chemokine oligomers by heparin. Here, we conducted a combined ion mobility-native mass spectrometry study to investigate the stability of chemokine oligomers and their complexes with fondaparinux, a synthetic heparin analog. Collision-induced dissociation and unfolding experiments provided clarity on the specificity and relevance of chemokine oligomers and their fondaparinux complexes with varying stoichiometries, as well as the stabilizing effects of fondaparinux binding.
Original language | English |
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Journal | Journal of the American Society for Mass Spectrometry |
Volume | 35 |
Issue number | 7 |
Pages (from-to) | 1550–1555 |
Number of pages | 6 |
ISSN | 1044-0305 |
DOIs | |
Publication status | Published - 2024 |
Bibliographical note
Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
ID: 395088401