Chemokine Oligomers and the Impact of Fondaparinux Binding
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Chemokine Oligomers and the Impact of Fondaparinux Binding. / Szekeres, Gergo Peter; Dyer, Douglas P.; Miller, Rebecca L.; Pagel, Kevin.
In: Journal of the American Society for Mass Spectrometry, Vol. 35, No. 7, 2024, p. 1550–1555.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Chemokine Oligomers and the Impact of Fondaparinux Binding
AU - Szekeres, Gergo Peter
AU - Dyer, Douglas P.
AU - Miller, Rebecca L.
AU - Pagel, Kevin
N1 - Publisher Copyright: © 2024 The Authors. Published by American Chemical Society.
PY - 2024
Y1 - 2024
N2 - Heparin, a widely used clinical anticoagulant, is generally well-tolerated; however, approximately 1% of patients develop heparin-induced thrombocytopenia (HIT), a serious side effect. While efforts to understand the role of chemokines in HIT development are ongoing, certain aspects remain less studied, such as the stabilization of chemokine oligomers by heparin. Here, we conducted a combined ion mobility-native mass spectrometry study to investigate the stability of chemokine oligomers and their complexes with fondaparinux, a synthetic heparin analog. Collision-induced dissociation and unfolding experiments provided clarity on the specificity and relevance of chemokine oligomers and their fondaparinux complexes with varying stoichiometries, as well as the stabilizing effects of fondaparinux binding.
AB - Heparin, a widely used clinical anticoagulant, is generally well-tolerated; however, approximately 1% of patients develop heparin-induced thrombocytopenia (HIT), a serious side effect. While efforts to understand the role of chemokines in HIT development are ongoing, certain aspects remain less studied, such as the stabilization of chemokine oligomers by heparin. Here, we conducted a combined ion mobility-native mass spectrometry study to investigate the stability of chemokine oligomers and their complexes with fondaparinux, a synthetic heparin analog. Collision-induced dissociation and unfolding experiments provided clarity on the specificity and relevance of chemokine oligomers and their fondaparinux complexes with varying stoichiometries, as well as the stabilizing effects of fondaparinux binding.
U2 - 10.1021/jasms.4c00142
DO - 10.1021/jasms.4c00142
M3 - Journal article
C2 - 38836362
AN - SCOPUS:85195286733
VL - 35
SP - 1550
EP - 1555
JO - Journal of The American Society for Mass Spectrometry
JF - Journal of The American Society for Mass Spectrometry
SN - 1044-0305
IS - 7
ER -
ID: 395088401