Cheirostylis takeoi, a new national species record from Mae Sai District, Chiang Rai Province, northern Thailand, is described, photographed and illustrated based on Thai material. Furthermore, we outline how it differs from the morphologically most similar species in Thailand, C. thailandica. The newly recorded species is mainly characterized by its narrowly oblong-lanceolate, obtuse to subacute, entire, strongly recurved labellum that lacks appendages inside the slightly concave base.