Cells exposed to a huntingtin fragment containing an expanded polyglutamine tract show no sign of ion channel formation: results arguing against the ion channel hypothesis.
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Cells exposed to a huntingtin fragment containing an expanded polyglutamine tract show no sign of ion channel formation: results arguing against the ion channel hypothesis. / Nørremølle, Anne; Grunnet, Morten; Hasholt, Lis; Sørensen, Sven Asger.
In: Journal of Neuroscience Research, Vol. 71, No. 1, 2003, p. 132-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cells exposed to a huntingtin fragment containing an expanded polyglutamine tract show no sign of ion channel formation: results arguing against the ion channel hypothesis.
AU - Nørremølle, Anne
AU - Grunnet, Morten
AU - Hasholt, Lis
AU - Sørensen, Sven Asger
N1 - Keywords: Animals; Blotting, Western; CHO Cells; Cricetinae; Green Fluorescent Proteins; Humans; Huntington Disease; Ion Channels; Luminescent Proteins; Membrane Potentials; Nerve Tissue Proteins; Nuclear Proteins; Oocytes; Patch-Clamp Techniques; Peptides; Recombinant Proteins; Time Factors; Transfection; Trinucleotide Repeat Expansion; Xenopus
PY - 2003
Y1 - 2003
N2 - Ion channels formed by expanded polyglutamine tracts have been proposed to play an important role in the pathological processes leading to neurodegeneration in Huntington's disease and other CAG repeat diseases. We tested the capacity of a huntingtin fragment containing an expanded polyglutamine tract to form ion channels in two cell types. Whole cell current from Xenopus oocytes was recorded using two-electrode voltage-clamp technique, and whole cell current from CHO-K1 cells was recorded by patch-clamp technique. The fragment with an expanded polyglutamine sequence induced no change in the currents recorded in any of the two expression systems, indicating no changes in ion channel activity. The results therefore argue against the proposed hypothesis of expanded polyglutamines forming ion channels.
AB - Ion channels formed by expanded polyglutamine tracts have been proposed to play an important role in the pathological processes leading to neurodegeneration in Huntington's disease and other CAG repeat diseases. We tested the capacity of a huntingtin fragment containing an expanded polyglutamine tract to form ion channels in two cell types. Whole cell current from Xenopus oocytes was recorded using two-electrode voltage-clamp technique, and whole cell current from CHO-K1 cells was recorded by patch-clamp technique. The fragment with an expanded polyglutamine sequence induced no change in the currents recorded in any of the two expression systems, indicating no changes in ion channel activity. The results therefore argue against the proposed hypothesis of expanded polyglutamines forming ion channels.
U2 - 10.1002/jnr.10468
DO - 10.1002/jnr.10468
M3 - Journal article
C2 - 12478622
VL - 71
SP - 132
EP - 137
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 1
ER -
ID: 8419052