Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats
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Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats. / Ghorbani, Marie Louise; Nyborg, Niels C B; Fjalland, Bjarne; Sheykhzade, Majid.
In: International Journal of Endocrinology, Vol. 2013, 2013, p. Article ID 532850.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Calcium activity of upper thoracic dorsal root ganglion neurons in zucker diabetic Fatty rats
AU - Ghorbani, Marie Louise
AU - Nyborg, Niels C B
AU - Fjalland, Bjarne
AU - Sheykhzade, Majid
PY - 2013
Y1 - 2013
N2 - The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca(2+)-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20¿mM¿K(+), followed by stimulation with either 0.3 or 0.5¿ µ M Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10(-5)¿M), and adenosine (10(-3)¿M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20¿mM¿K(+) compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.
AB - The aim of the present study was to examine the calcium activity of C8-T5 dorsal root ganglion (DRG) neurons from Zucker diabetic fatty rats. In total, 8 diabetic ZDF fatty animals and 8 age-matched control ZDF lean rats were employed in the study. C8-T5 dorsal root ganglia were isolated bilaterally from 14 to 18 weeks old rats, and a primary culture was prepared. Calcium activity was measured ratiometrically using the fluorescent Ca(2+)-indicator Fura-2 acetoxymethyl ester. All neurons were stimulated twice with 20¿mM¿K(+), followed by stimulation with either 0.3 or 0.5¿ µ M Capsaicin, alone or in combination with algogenic chemicals (bradykinin, serotonin, prostaglandin E2 (all 10(-5)¿M), and adenosine (10(-3)¿M)) at pH 7.4 and 6.0. Neurons from diabetic animals exhibited an overall increased response to stimulation with 20¿mM¿K(+) compared to neurons from control. Stimulation with Capsaicin alone caused an augmented response in neurons from diabetic animals compared to control animals. When stimulated with a combination of Capsaicin and algogenic chemicals, no differences between the two groups of neurons were measured, neither at pH 7.4 nor 6.0. In conclusion, diabetes-induced alterations in calcium activity of the DRG neurons were found, potentially indicating altered neuronal responses during myocardial ischemia.
U2 - 10.1155/2013/532850
DO - 10.1155/2013/532850
M3 - Journal article
C2 - 23662103
VL - 2013
SP - Article ID 532850
JO - International Journal of Endocrinology
JF - International Journal of Endocrinology
SN - 1687-8337
ER -
ID: 45665493