Brain uptake of alpha-[14C]methyl-para-tyrosine in the rat.
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Brain uptake of alpha-[14C]methyl-para-tyrosine in the rat. / Cumming, P; Venkatachalam, T K; Rajagopal, S; Diksic, M; Gjedde, A.
In: Synapse, Vol. 17, No. 2, 1994, p. 125-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Brain uptake of alpha-[14C]methyl-para-tyrosine in the rat.
AU - Cumming, P
AU - Venkatachalam, T K
AU - Rajagopal, S
AU - Diksic, M
AU - Gjedde, A
PY - 1994
Y1 - 1994
N2 - The blood-brain permeabilities of L-[3H]tyrosine and the tyrosine hydroxylase (TH) inhibitor alpha-[14C]methyl-para-tyrosine ([14C]AMPT) were determined in rat striatum, a brain region rich in TH activity, and in other brain regions containing relatively little TH activity. In striatum, the unidirectional clearance rate (K1) for L-[3H]tyrosine (6.2 ml hg-1 min-1) was significantly greater than the rates for L-[14C]AMPT (2.8 ml hg-1 min-1) and D-[14C]AMPT (0.8 ml hg-1 min-1). The apparent volume of distribution (Vf) for L-[14C]AMPT in striatum (72.5 +/- 4.0 ml hg-1) did not differ from the Vf in other brain regions. The homogeneous distribution of L-[14C]AMPT in rat brain indicates that labeled AMPT is unsuitable for the study of TH in vivo by quantitative autoradiography.
AB - The blood-brain permeabilities of L-[3H]tyrosine and the tyrosine hydroxylase (TH) inhibitor alpha-[14C]methyl-para-tyrosine ([14C]AMPT) were determined in rat striatum, a brain region rich in TH activity, and in other brain regions containing relatively little TH activity. In striatum, the unidirectional clearance rate (K1) for L-[3H]tyrosine (6.2 ml hg-1 min-1) was significantly greater than the rates for L-[14C]AMPT (2.8 ml hg-1 min-1) and D-[14C]AMPT (0.8 ml hg-1 min-1). The apparent volume of distribution (Vf) for L-[14C]AMPT in striatum (72.5 +/- 4.0 ml hg-1) did not differ from the Vf in other brain regions. The homogeneous distribution of L-[14C]AMPT in rat brain indicates that labeled AMPT is unsuitable for the study of TH in vivo by quantitative autoradiography.
U2 - 10.1002/syn.890170208
DO - 10.1002/syn.890170208
M3 - Journal article
C2 - 7916491
VL - 17
SP - 125
EP - 128
JO - Synapse
JF - Synapse
SN - 0887-4476
IS - 2
ER -
ID: 14943177