Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta
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Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta. / Jinkerson, Robert E.; Poveda-Huertes, Daniel; Cooney, Elizabeth C.; Cho, Anna; Ochoa-Fernandez, Rocio; Keeling, Patrick J.; Xiang, Tingting; Andersen-Ranberg, Johan.
In: Current Biology, Vol. 34, No. 3, 2024, p. 594-605.e4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Biosynthesis of chlorophyll c in a dinoflagellate and heterologous production in planta
AU - Jinkerson, Robert E.
AU - Poveda-Huertes, Daniel
AU - Cooney, Elizabeth C.
AU - Cho, Anna
AU - Ochoa-Fernandez, Rocio
AU - Keeling, Patrick J.
AU - Xiang, Tingting
AU - Andersen-Ranberg, Johan
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2024
Y1 - 2024
N2 - Chlorophyll c is a key photosynthetic pigment that has been used historically to classify eukaryotic algae. Despite its importance in global photosynthetic productivity, the pathway for its biosynthesis has remained elusive. Here we define the CHLOROPHYLL C SYNTHASE (CHLCS) discovered through investigation of a dinoflagellate mutant deficient in chlorophyll c. CHLCSs are proteins with chlorophyll a/b binding and 2-oxoglutarate-Fe(II) dioxygenase (2OGD) domains found in peridinin-containing dinoflagellates; other chlorophyll c-containing algae utilize enzymes with only the 2OGD domain or an unknown synthase to produce chlorophyll c. 2OGD-containing synthases across dinoflagellate, diatom, cryptophyte, and haptophyte lineages form a monophyletic group, 8 members of which were also shown to produce chlorophyll c. Chlorophyll c1 to c2 ratios in marine algae are dictated in part by chlorophyll c synthases. CHLCS heterologously expressed in planta results in the accumulation of chlorophyll c1 and c2, demonstrating a path to augment plant pigment composition with algal counterparts.
AB - Chlorophyll c is a key photosynthetic pigment that has been used historically to classify eukaryotic algae. Despite its importance in global photosynthetic productivity, the pathway for its biosynthesis has remained elusive. Here we define the CHLOROPHYLL C SYNTHASE (CHLCS) discovered through investigation of a dinoflagellate mutant deficient in chlorophyll c. CHLCSs are proteins with chlorophyll a/b binding and 2-oxoglutarate-Fe(II) dioxygenase (2OGD) domains found in peridinin-containing dinoflagellates; other chlorophyll c-containing algae utilize enzymes with only the 2OGD domain or an unknown synthase to produce chlorophyll c. 2OGD-containing synthases across dinoflagellate, diatom, cryptophyte, and haptophyte lineages form a monophyletic group, 8 members of which were also shown to produce chlorophyll c. Chlorophyll c1 to c2 ratios in marine algae are dictated in part by chlorophyll c synthases. CHLCS heterologously expressed in planta results in the accumulation of chlorophyll c1 and c2, demonstrating a path to augment plant pigment composition with algal counterparts.
KW - 2 oxoglutarate-Fe(II) dioxygenase
KW - biosynthesis
KW - Breviolum minutum
KW - CHLCS
KW - chlorophyll c
KW - CHLOROPHYLL C SYNTHASE
KW - chromalveolate
KW - dinoflagellate
U2 - 10.1016/j.cub.2023.12.068
DO - 10.1016/j.cub.2023.12.068
M3 - Journal article
C2 - 38157859
AN - SCOPUS:85181254635
VL - 34
SP - 594-605.e4
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 3
ER -
ID: 383748301