Aquaglyceroporins and orthodox aquaporins in human adipocytes
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Aquaglyceroporins and orthodox aquaporins in human adipocytes. / Huang, Peng; Hansen, Jesper S; Saba, Karim H; Bergman, Anna; Negoita, Florentina; Gourdon, Pontus; Hagström-Andersson, Anna; Lindkvist-Petersson, Karin.
In: B B A - Biomembranes, Vol. 1864, No. 1, 183795, 01.02.2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Aquaglyceroporins and orthodox aquaporins in human adipocytes
AU - Huang, Peng
AU - Hansen, Jesper S
AU - Saba, Karim H
AU - Bergman, Anna
AU - Negoita, Florentina
AU - Gourdon, Pontus
AU - Hagström-Andersson, Anna
AU - Lindkvist-Petersson, Karin
N1 - Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquaglyceroporins, we discovered by transcriptome sequencing that AQP1 is expressed in human primary adipocytes. AQP1 is mainly a water channel and thus is thought to be involved in the response to hyper-osmotic stress by efflux of water during hyperglycemia. Thus, this data suggests a contribution of both orthodox aquaporin and aquaglyceroporin in human adipocytes to maintain the homeostasis of glycerol and water during fasting and feeding.
AB - Aquaporins play a crucial role in water homeostasis in the human body, and recently the physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 and AQP10) represent key glycerol channels, enabling glycerol flux across the membranes of cells. Adipocytes are the major source of glycerol and during lipolysis, glycerol is released to be metabolized by other tissues through a well-orchestrated process. Here we show that both AQP3 and AQP7 bind to the lipid droplet protein perilipin 1 (PLIN1), suggesting that PLIN1 is involved in the coordination of the subcellular translocation of aquaglyceroporins in human adipocytes. Moreover, in addition to aquaglyceroporins, we discovered by transcriptome sequencing that AQP1 is expressed in human primary adipocytes. AQP1 is mainly a water channel and thus is thought to be involved in the response to hyper-osmotic stress by efflux of water during hyperglycemia. Thus, this data suggests a contribution of both orthodox aquaporin and aquaglyceroporin in human adipocytes to maintain the homeostasis of glycerol and water during fasting and feeding.
KW - Adipocytes/metabolism
KW - Aquaglyceroporins/genetics
KW - Aquaporin 1/genetics
KW - Aquaporin 3/genetics
KW - Aquaporins/genetics
KW - Gene Expression Regulation/genetics
KW - Glycerol/metabolism
KW - Homeostasis/genetics
KW - Humans
KW - Hyperglycemia/genetics
KW - Perilipin-1/genetics
KW - Transcriptome/genetics
KW - Water/metabolism
U2 - 10.1016/j.bbamem.2021.183795
DO - 10.1016/j.bbamem.2021.183795
M3 - Journal article
C2 - 34627746
VL - 1864
JO - B B A - Biomembranes
JF - B B A - Biomembranes
SN - 0005-2736
IS - 1
M1 - 183795
ER -
ID: 338428991