TY - JOUR

T1 - All Staphylococcus aureus bacteraemia-inducing strains can cause infective endocarditis

T2 - Results of GWAS and experimental animal studies

AU - Bastien, Sylvère

AU - Meyers, Severien

AU - Salgado-Pabón, Wilmara

AU - Giulieri, Stefano G.

AU - Rasigade, Jean-Phillipe

AU - Liesenborghs, Laurens

AU - Kinney, Kyle J.

AU - Couzon, Florence

AU - Martins-Simoes, Patricia

AU - Moing, Vincent Le

AU - Duval, Xavier

AU - Holmes, Natasha E.

AU - Bruun, Niels Eske

AU - Skov, Robert

AU - Howden, Benjamin P.

AU - Fowler, Vance G.

AU - Verhamme, Peter

AU - Andersen, Paal Skytt

AU - Bouchiat, Coralie

AU - Moreau, Karen

AU - Vandenesch, François

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - Objectives: We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB). Methods: A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves. Results: The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU. Conclusion: All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.

AB - Objectives: We aimed at determining whether specific S. aureus strains cause infective endocarditis (IE) in the course of Staphylococcus aureus bacteraemia (SAB). Methods: A genome-wide association study (GWAS) including 924 S. aureus genomes from IE (274) and non-IE (650) SAB patients from international cohorts was conducted, and a subset of strains was tested with two experimental animal models of IE, one investigating the early step of bacterial adhesion to inflamed mice valves, the second evaluating the local and systemic developmental process of IE on mechanically-damaged rabbit valves. Results: The genetic profile of S. aureus IE and non-IE SAB strains did not differ when considering single nucleotide polymorphisms, coding sequences, and k-mers analysed in GWAS. In the murine inflammation-induced IE model, no difference was observed between IE and non-IE SAB strains both in terms of adhesion to the cardiac valves and in the propensity to cause IE; in the mechanical IE-induced rabbit model, there was no difference between IE and non-IE SAB strains regarding the vegetation size and CFU. Conclusion: All strains of S. aureus isolated from SAB patients must be considered as capable of causing this common and lethal infection once they have accessed the bloodstream.

KW - Bacteraemia

KW - Experimental animal model

KW - Genome-wide association study

KW - Infective endocarditis

KW - Staphylococcus aureus

U2 - 10.1016/j.jinf.2022.12.028

DO - 10.1016/j.jinf.2022.12.028

M3 - Journal article

C2 - 36603774

AN - SCOPUS:85146001645

VL - 86

SP - 123

EP - 133

JO - Journal of Infection

JF - Journal of Infection

SN - 0163-4453

IS - 2

ER -