A suction blister protocol to study human Tk-cell recall responses in vivo
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- A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
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Cutaneous antigen-recall models allow for studies of human memory responses in vivo. When combined with skin suction blister (SB) induction, this model offers accessibility to rare populations of antigen-specific T-cells representative of the cellular memory response as well as the cytokine microenvironment in situ. This report describes the practical procedure of a cutaneous recall, an SB induction, and a harvest of antigen-specific T-cells. To exemplify the method, the tuberculin skin test is used for antigenic recall in individuals who, prior to this study, underwent a Bacillus Calmette-Guérin vaccination against an infection with Mycobacterium tuberculosis. Finally, examples of multiplex and flow cytometric analyses of SB specimens are provided, illustrating high fractions of antigen-specific polyfunctional CD4+ T-cells available by this sampling method compared with cells isolated from the blood. The method described here is safe and minimally invasive, provides a unique opportunity to study both innate and adaptive immune responses in vivo, and may be beneficial to a broad community of researchers working with cell-mediated immunity and human memory responses, in the context of vaccine development.
Original language | English |
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Article number | e57554 |
Journal | Journal of Visualized Experiments |
Volume | 2018 |
Issue number | 138 |
Number of pages | 8 |
ISSN | 1940-087X |
DOIs | |
Publication status | Published - 2018 |
- Antigen, Immunology, Immunology and infection, In vivo response, Issue 138, Memory T-cell, PPD, Recall, Skin challenge, Suction blister, T-cell, Tuberculin skin test, Tuberculosis, Vaccines
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