A simple method for validation and verification of pipettes mounted on automated liquid handlers
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A simple method for validation and verification of pipettes mounted on automated liquid handlers. / Stangegaard, Michael; Hansen, Anders Johannes; Frøslev, Tobias G; Morling, Niels.
In: JALA - Journal of the Association for Laboratory Automation, Vol. 16, No. 5, 01.10.2011, p. 381-386.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A simple method for validation and verification of pipettes mounted on automated liquid handlers
AU - Stangegaard, Michael
AU - Hansen, Anders Johannes
AU - Frøslev, Tobias G
AU - Morling, Niels
N1 - Copyright © 2011 Society for Laboratory Automation and Screening. Published by Elsevier Inc. All rights reserved.
PY - 2011/10/1
Y1 - 2011/10/1
N2 - We have implemented a simple, inexpensive, and fast procedure for validation and verification of the performance of pipettes mounted on automated liquid handlers (ALHs) as necessary for laboratories accredited under ISO 17025. A six- or seven-step serial dilution of OrangeG was prepared in quadruplicates in a flat-bottom 96-well microtiter plate, manually using calibrated pipettes. Each pipette of the liquid handler (1-8) dispensed a selected volume (1-200 µL) of OrangeG eight times into the wells of the microtiter plate. All wells contained a total of 200 µL liquid. The absorbance was read, and the dispensed volume of each pipette was calculated based on a plot of volume and absorbance of a known set of OrangeG dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette were calculated. Using predefined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid-handler software. We have implemented the procedure on a Sias Xantus, an MWGt TheONYX, four Tecan Freedom EVO, a Biomek NX Span-8, and four Biomek 3000 robots, and the methods are freely available. In conclusion, we have set up a simple, inexpensive, and fast solution for the continuous validation of ALHs used for accredited work according to the ISO 17025 standard. The method is easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.
AB - We have implemented a simple, inexpensive, and fast procedure for validation and verification of the performance of pipettes mounted on automated liquid handlers (ALHs) as necessary for laboratories accredited under ISO 17025. A six- or seven-step serial dilution of OrangeG was prepared in quadruplicates in a flat-bottom 96-well microtiter plate, manually using calibrated pipettes. Each pipette of the liquid handler (1-8) dispensed a selected volume (1-200 µL) of OrangeG eight times into the wells of the microtiter plate. All wells contained a total of 200 µL liquid. The absorbance was read, and the dispensed volume of each pipette was calculated based on a plot of volume and absorbance of a known set of OrangeG dilutions. Finally, the percent inaccuracy (%d) and the imprecision (%CV) of each pipette were calculated. Using predefined acceptance criteria, each pipette was then either approved or failed. Failed pipettes were either repaired or the volume deviation was compensated for by applying a calibration curve in the liquid-handler software. We have implemented the procedure on a Sias Xantus, an MWGt TheONYX, four Tecan Freedom EVO, a Biomek NX Span-8, and four Biomek 3000 robots, and the methods are freely available. In conclusion, we have set up a simple, inexpensive, and fast solution for the continuous validation of ALHs used for accredited work according to the ISO 17025 standard. The method is easy to use for aqueous solutions but requires a spectrophotometer that can read microtiter plates.
U2 - 10.1016/j.jala.2009.06.004
DO - 10.1016/j.jala.2009.06.004
M3 - Journal article
C2 - 21906565
VL - 16
SP - 381
EP - 386
JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation
JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation
SN - 2211-0682
IS - 5
ER -
ID: 35353244