A Colloid Approach to Self-Assembling Antibodies
Research output: Contribution to journal › Journal article › Research › peer-review
Concentrated solutions of monoclonal antibodies have attracted considerable attention due to their importance in pharmaceutical formulations; yet, their tendency to aggregate and the resulting high viscosity pose considerable problems. Here we tackle this problem by a soft condensed matter physics approach, which combines a variety of experimental measurements with a patchy colloid model, amenable of analytical solution. We thus report results of structural antibodies and dynamic properties obtained through scattering methods and microrheological experiments. We model the data using a colloid-inspired approach, explicitly taking into account both the anisotropic shape of the molecule and its charge distribution. Our simple patchy model is able to disentangle self-assembly and intermolecular interactions and to quantitatively describe the concentration-dependence of the osmotic compressibility, collective diffusion coefficient, and zero shear viscosity. Our results offer new insights on the key problem of antibody formulations, providing a theoretical and experimental framework for a quantitative assessment of the effects of additional excipients or chemical modifications and a prediction of the resulting viscosity.
Original language | English |
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Journal | Molecular Pharmaceutics |
Volume | 16 |
Issue number | 6 |
Pages (from-to) | 2394-2404 |
Number of pages | 11 |
ISSN | 1543-8384 |
DOIs | |
Publication status | Published - 3 Jun 2019 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:
© 2019 American Chemical Society.
- antibodies, patchy colloids, self-assembly
Research areas
ID: 367842187