Renal uptake and excretion of epidermal growth factor from plasma in the rat
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Renal uptake and excretion of epidermal growth factor from plasma in the rat. / Jørgensen, Per E.; Rasmussen, Torben N.; Olsen, Peter Skov; Raaberg, Lasse; Poulsen, Steen Seier; Nexø, Ebba.
I: Regulatory Peptides, Bind 28, Nr. 3, 21.05.1990, s. 273-280.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Renal uptake and excretion of epidermal growth factor from plasma in the rat
AU - Jørgensen, Per E.
AU - Rasmussen, Torben N.
AU - Olsen, Peter Skov
AU - Raaberg, Lasse
AU - Poulsen, Steen Seier
AU - Nexø, Ebba
PY - 1990/5/21
Y1 - 1990/5/21
N2 - The rat excretes around 2 nmol epidermal growth factor (EGF) in the urine per 24 h. The urinary EGF might be derived from plasma and/or might be synthesized in the kidneys. We have used the rat to study the renal uptake and excretion of homologous EGF from plasma. I.v. injected 125I-EGF was removed from the circulation within a few minutes. 5 min after the injection, the kidneys contained 12% of the 125I-EGF. The kidneys seemed to degrade most of the 125I-EGF which they accumulated from blood, as only 4% of the injected label was excreted as intact 125I-EGF in the urine. The amount of endogenous EGF in plasma was under the detection limit of our enzyme-linked immunosorbent assay (0.03 nmol/l) and it remained so after bilateral nephrectomy. Even if plasma EGF was 0.03 nmol/l excretion of EGF from plasma could account for less than 5% of the urinary EGF. This study shows that the kidneys are able to accumulate EGF from plasma and excrete a part of it as intact EGF in the urine. However, excretion of immunoreactive EGF from plasma can only account for a minor part of the urinary EGF.
AB - The rat excretes around 2 nmol epidermal growth factor (EGF) in the urine per 24 h. The urinary EGF might be derived from plasma and/or might be synthesized in the kidneys. We have used the rat to study the renal uptake and excretion of homologous EGF from plasma. I.v. injected 125I-EGF was removed from the circulation within a few minutes. 5 min after the injection, the kidneys contained 12% of the 125I-EGF. The kidneys seemed to degrade most of the 125I-EGF which they accumulated from blood, as only 4% of the injected label was excreted as intact 125I-EGF in the urine. The amount of endogenous EGF in plasma was under the detection limit of our enzyme-linked immunosorbent assay (0.03 nmol/l) and it remained so after bilateral nephrectomy. Even if plasma EGF was 0.03 nmol/l excretion of EGF from plasma could account for less than 5% of the urinary EGF. This study shows that the kidneys are able to accumulate EGF from plasma and excrete a part of it as intact EGF in the urine. However, excretion of immunoreactive EGF from plasma can only account for a minor part of the urinary EGF.
KW - Blood
KW - Epidermal growth factor
KW - Kidney
KW - Metabolism
KW - Rat
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=0025231281&partnerID=8YFLogxK
U2 - 10.1016/0167-0115(90)90025-R
DO - 10.1016/0167-0115(90)90025-R
M3 - Journal article
C2 - 2377743
AN - SCOPUS:0025231281
VL - 28
SP - 273
EP - 280
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 3
ER -
ID: 199324358