Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli
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Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli. / Krishna, Sandeep; Orosz, László; Sneppen, Kim; Adhya, Sankar; Semsey, Szabolcs.
I: Journal of Molecular Biology, Bind 391, Nr. 4, 2009, s. 671-678.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Relation of intracellular signal levels and promoter activities in the gal regulon of escherichia coli
AU - Krishna, Sandeep
AU - Orosz, László
AU - Sneppen, Kim
AU - Adhya, Sankar
AU - Semsey, Szabolcs
PY - 2009
Y1 - 2009
N2 - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.
AB - Transcription of many genes is regulated by combinations of multiple signals. In Escherichia coli, combinatorial control is typical in the case of operons related to utilization of different sugars in the absence of glucose. To understand regulation of the transport and metabolic pathways in the galactose system, we measured activities of the six gal regulon promoters simultaneously, using an in vitro transcription system containing purified components. Input functions were computed on the basis of the experimental measurements. We observed four different shapes of input functions. From the results, we can conclude that the structure of the regulatory network is insufficient for the determination of signal integration. It is the actual structure of the promoter and regulatory region, the mechanism of transcription regulation, and the interplay between transcription factors that shape the input function to be suitable for adaptation.
KW - Escherichia coli
KW - Escherichia coli Proteins
KW - Galactose
KW - Gene Expression Regulation, Bacterial
KW - Promoter Regions, Genetic
KW - Regulon
KW - Signal Transduction
KW - Transcription Factors
KW - Journal Article
KW - Research Support, N.I.H., Intramural
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.jmb.2009.06.043
DO - 10.1016/j.jmb.2009.06.043
M3 - Journal article
C2 - 19559028
VL - 391
SP - 671
EP - 678
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 4
ER -
ID: 17496525