Peptide-evoked release of amylase from isolated acini of the rat parotid gland
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Peptide-evoked release of amylase from isolated acini of the rat parotid gland. / Goll, R; Poulsen, J H; Schmidt, P; Schjoldager, B; Poulsen, Steen Seier; Holst, J J.
I: Regulatory Peptides, Bind 51, Nr. 3, 26.05.1994, s. 237-54.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Peptide-evoked release of amylase from isolated acini of the rat parotid gland
AU - Goll, R
AU - Poulsen, J H
AU - Schmidt, P
AU - Schjoldager, B
AU - Poulsen, Steen Seier
AU - Holst, J J
PY - 1994/5/26
Y1 - 1994/5/26
N2 - Investigations of the effects of the neuropeptides, substance P (SP), neurokinin A (NKA), neuropeptide K (NPK), gastrin releasing peptide (GRP), calcitonin gene related peptide (CGRP) and vasoactive intestinal peptide (VIP), and of acetylcholine on amylase secretion have been carried out on isolated acini of the rat parotid gland. Furthermore, the occurrence and location of the peptides in the gland was studied. Finally, binding of 125I-BH-SP to isolated acini were studied in order to characterize their tachykinin receptor(s) and their binding kinetics. Only SP, NKA, NPK and VIP stimulated amylase release. VIP, however, with a rather low potency (EC50 at 155 nmol/l). Simultaneous stimulation with two compounds elicited additive responses, except for VIP and acetylcholine which elicited an effect significantly above additive response. Only SP, NKA, VIP and CGRP could be identified in extracts of the gland. The immunoreactivity of these peptides could be located to varicose nerve fibers in the gland. Binding of labeled SP to the isolated acini exhibited the characteristics of a genuine agonist/receptor interaction, and the rank order of displacement potencies indicated the presence of NK1-receptors. Thus, the results of the present study support previous suggestions that the tachykinins and VIP are likely to be involved in amylase secretion in the rat parotid gland.
AB - Investigations of the effects of the neuropeptides, substance P (SP), neurokinin A (NKA), neuropeptide K (NPK), gastrin releasing peptide (GRP), calcitonin gene related peptide (CGRP) and vasoactive intestinal peptide (VIP), and of acetylcholine on amylase secretion have been carried out on isolated acini of the rat parotid gland. Furthermore, the occurrence and location of the peptides in the gland was studied. Finally, binding of 125I-BH-SP to isolated acini were studied in order to characterize their tachykinin receptor(s) and their binding kinetics. Only SP, NKA, NPK and VIP stimulated amylase release. VIP, however, with a rather low potency (EC50 at 155 nmol/l). Simultaneous stimulation with two compounds elicited additive responses, except for VIP and acetylcholine which elicited an effect significantly above additive response. Only SP, NKA, VIP and CGRP could be identified in extracts of the gland. The immunoreactivity of these peptides could be located to varicose nerve fibers in the gland. Binding of labeled SP to the isolated acini exhibited the characteristics of a genuine agonist/receptor interaction, and the rank order of displacement potencies indicated the presence of NK1-receptors. Thus, the results of the present study support previous suggestions that the tachykinins and VIP are likely to be involved in amylase secretion in the rat parotid gland.
KW - Acetylcholine
KW - Amylases
KW - Animals
KW - Calcitonin Gene-Related Peptide
KW - Fluorescent Antibody Technique
KW - Gastrin-Releasing Peptide
KW - Male
KW - Nerve Fibers
KW - Neurokinin A
KW - Neuropeptides
KW - Parotid Gland
KW - Peptides
KW - Radioimmunoassay
KW - Rats
KW - Rats, Wistar
KW - Receptors, Neurokinin-1
KW - Substance P
KW - Tachykinins
KW - Vasoactive Intestinal Peptide
M3 - Journal article
C2 - 7524117
VL - 51
SP - 237
EP - 254
JO - Regulatory Peptides
JF - Regulatory Peptides
SN - 0167-0115
IS - 3
ER -
ID: 47487201