Optimized, fast through-put UHPLC-DAD based method for carotenoid quantification in spinach, serum, chylomicrons and faeces
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Optimized, fast through-put UHPLC-DAD based method for carotenoid quantification in spinach, serum, chylomicrons and faeces. / Eriksen, Jane Nygaard; Madsen, Pia Lisbeth; Dragsted, Lars Ove; Arrigoni, Eva.
I: Journal of Agricultural and Food Chemistry, Bind 65, Nr. 4, 2017, s. 973-980.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Optimized, fast through-put UHPLC-DAD based method for carotenoid quantification in spinach, serum, chylomicrons and faeces
AU - Eriksen, Jane Nygaard
AU - Madsen, Pia Lisbeth
AU - Dragsted, Lars Ove
AU - Arrigoni, Eva
N1 - CURIS 2017 NEXS 030
PY - 2017
Y1 - 2017
N2 - An improved UHPLC-DAD based method was developed and validated for quantification of major carotenoids present in spinach, serum, chylomicrons and faeces. Separation was achieved with gradient elution within 12.5 min for 6 dietary carotenoids and the internal standard, echinenone. The proposed method provides, for all standard components, resolution >1.1, linearity covering the target range (R>0.99), LOQ <0.035 mg/L, intra-day and inter-day RSD <2% and 10%, respectively. Suitability of the method was tested on biological matrices. Method precision (RSD%) for carotenoid quantification in serum, chylomicrons and faeces was below 10% for intra- and inter-day analysis, except for lycopene. Method accuracy was consistent with mean recoveries ranging from 78.8-96.9 and 57.2-96.9% for all carotenoids, except for lycopene, in serum and faeces, respectively. Additionally, an inter-laboratory validation study on spinach at two institutions showed no significant differences in lutein or β-carotene content, when evaluated at 4 occasions.
AB - An improved UHPLC-DAD based method was developed and validated for quantification of major carotenoids present in spinach, serum, chylomicrons and faeces. Separation was achieved with gradient elution within 12.5 min for 6 dietary carotenoids and the internal standard, echinenone. The proposed method provides, for all standard components, resolution >1.1, linearity covering the target range (R>0.99), LOQ <0.035 mg/L, intra-day and inter-day RSD <2% and 10%, respectively. Suitability of the method was tested on biological matrices. Method precision (RSD%) for carotenoid quantification in serum, chylomicrons and faeces was below 10% for intra- and inter-day analysis, except for lycopene. Method accuracy was consistent with mean recoveries ranging from 78.8-96.9 and 57.2-96.9% for all carotenoids, except for lycopene, in serum and faeces, respectively. Additionally, an inter-laboratory validation study on spinach at two institutions showed no significant differences in lutein or β-carotene content, when evaluated at 4 occasions.
KW - Faculty of Science
KW - UHPLC-DAD
KW - Lutein
KW - Beta-carotene
KW - Green leafy vegetables
KW - Human samples
U2 - 10.1021/acs.jafc.6b04925
DO - 10.1021/acs.jafc.6b04925
M3 - Journal article
C2 - 28004571
VL - 65
SP - 973
EP - 980
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 4
ER -
ID: 171118894