One-Tip enables comprehensive proteome coverage in minimal cells and single zygotes: [Inkl. Correction]
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Dokumenter
- Fulltext
Forlagets udgivne version, 1,8 MB, PDF-dokument
Mass spectrometry (MS)-based proteomics workflows typically involve complex, multi-step processes, presenting challenges with sample losses, reproducibility, requiring substantial time and financial investments, and specialized skills. Here we introduce One-Tip, a proteomics methodology that seamlessly integrates efficient, one-pot sample preparation with precise, narrow-window data-independent acquisition (nDIA) analysis. One-Tip substantially simplifies sample processing, enabling the reproducible identification of >9000 proteins from ~1000 HeLa cells. The versatility of One-Tip is highlighted by nDIA identification of ~6000 proteins in single cells from early mouse embryos. Additionally, the study incorporates the Uno Single Cell Dispenser™, demonstrating the capability of One-Tip in single-cell proteomics with >3000 proteins identified per HeLa cell. We also extend One-Tip workflow to analysis of extracellular vesicles (EVs) extracted from blood plasma, demonstrating its high sensitivity by identifying >3000 proteins from 16 ng EV preparation. One-Tip expands capabilities of proteomics, offering greater depth and throughput across a range of sample types.
Originalsprog | Engelsk |
---|---|
Artikelnummer | 2474 |
Tidsskrift | Nature Communications |
Vol/bind | 15 |
Udgave nummer | 1 |
Antal sider | 10 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2024 |
Bibliografisk note
Publisher Copyright:
© The Author(s) 2024.
Antal downloads er baseret på statistik fra Google Scholar og www.ku.dk
ID: 387020946