Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry
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Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry. / Hatse, Sigrid; Princen, Katrien; Vermeire, Kurt; Gerlach, Lars-Ole; Rosenkilde, Mette M; Schwartz, Thue W; Bridger, Gary; De Clercq, Erik; Schols, Dominique.
I: FEBS Letters, Bind 546, Nr. 2-3, 2003, s. 300-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mutations at the CXCR4 interaction sites for AMD3100 influence anti-CXCR4 antibody binding and HIV-1 entry
AU - Hatse, Sigrid
AU - Princen, Katrien
AU - Vermeire, Kurt
AU - Gerlach, Lars-Ole
AU - Rosenkilde, Mette M
AU - Schwartz, Thue W
AU - Bridger, Gary
AU - De Clercq, Erik
AU - Schols, Dominique
N1 - Keywords: Amino Acid Sequence; Binding Sites; Flow Cytometry; HIV-1; Heterocyclic Compounds; Humans; Membrane Fusion; Molecular Sequence Data; Mutation; Receptors, CXCR4; Tumor Cells, Cultured
PY - 2003
Y1 - 2003
N2 - The interaction of the CXCR4 antagonist AMD3100 with its target is greatly influenced by specific aspartate residues in the receptor protein, including Asp(171) and Asp(262). We have now found that aspartate-to-asparagine substitutions at these positions differentially affect the binding of four different anti-CXCR4 monoclonal antibodies as well as the infectivity of diverse human immunodeficiency virus type 1 (HIV-1) strains and clinical isolates. Mutation of Asp(262) strongly decreased the coreceptor efficiency of CXCR4 for wild-type but not for AMD3100-resistant HIV-1 NL4.3. Thus, resistance of HIV-1 NL4.3 to AMD3100 is associated with a decreased dependence of the viral gp120 on Asp(262) of CXCR4, pointing to a different mode of interaction of wild-type versus AMD3100-resistant virus with CXCR4.
AB - The interaction of the CXCR4 antagonist AMD3100 with its target is greatly influenced by specific aspartate residues in the receptor protein, including Asp(171) and Asp(262). We have now found that aspartate-to-asparagine substitutions at these positions differentially affect the binding of four different anti-CXCR4 monoclonal antibodies as well as the infectivity of diverse human immunodeficiency virus type 1 (HIV-1) strains and clinical isolates. Mutation of Asp(262) strongly decreased the coreceptor efficiency of CXCR4 for wild-type but not for AMD3100-resistant HIV-1 NL4.3. Thus, resistance of HIV-1 NL4.3 to AMD3100 is associated with a decreased dependence of the viral gp120 on Asp(262) of CXCR4, pointing to a different mode of interaction of wild-type versus AMD3100-resistant virus with CXCR4.
M3 - Journal article
C2 - 12832058
VL - 546
SP - 300
EP - 306
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 2-3
ER -
ID: 78189