Linking thermodynamics and measurements of protein stability

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Standard

Linking thermodynamics and measurements of protein stability. / Lindorff-Larsen, Kresten; Teilum, Kaare.

I: Protein engineering, design & selection : PEDS, Bind 34, gzab002, 2021.

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Harvard

Lindorff-Larsen, K & Teilum, K 2021, 'Linking thermodynamics and measurements of protein stability', Protein engineering, design & selection : PEDS, bind 34, gzab002. https://doi.org/10.1093/protein/gzab002

APA

Lindorff-Larsen, K., & Teilum, K. (2021). Linking thermodynamics and measurements of protein stability. Protein engineering, design & selection : PEDS, 34, [gzab002]. https://doi.org/10.1093/protein/gzab002

Vancouver

Lindorff-Larsen K, Teilum K. Linking thermodynamics and measurements of protein stability. Protein engineering, design & selection : PEDS. 2021;34. gzab002. https://doi.org/10.1093/protein/gzab002

Author

Lindorff-Larsen, Kresten ; Teilum, Kaare. / Linking thermodynamics and measurements of protein stability. I: Protein engineering, design & selection : PEDS. 2021 ; Bind 34.

Bibtex

@article{adc9b1e324f648468f0a4e2c1e776b28,
title = "Linking thermodynamics and measurements of protein stability",
abstract = "We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible folding/unfolding transitions and the experimental methods that are available for extracting thermodynamic parameters. We highlight the importance of modelling both how the folding equilibrium depends on a perturbing variable such as temperature or denaturant concentration, and the importance of modelling the baselines in the experimental observables.",
author = "Kresten Lindorff-Larsen and Kaare Teilum",
year = "2021",
doi = "10.1093/protein/gzab002",
language = "English",
volume = "34",
journal = "Protein Engineering, Design and Selection",
issn = "1741-0126",
publisher = "Oxford University Press",

}

RIS

TY - JOUR

T1 - Linking thermodynamics and measurements of protein stability

AU - Lindorff-Larsen, Kresten

AU - Teilum, Kaare

PY - 2021

Y1 - 2021

N2 - We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible folding/unfolding transitions and the experimental methods that are available for extracting thermodynamic parameters. We highlight the importance of modelling both how the folding equilibrium depends on a perturbing variable such as temperature or denaturant concentration, and the importance of modelling the baselines in the experimental observables.

AB - We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible folding/unfolding transitions and the experimental methods that are available for extracting thermodynamic parameters. We highlight the importance of modelling both how the folding equilibrium depends on a perturbing variable such as temperature or denaturant concentration, and the importance of modelling the baselines in the experimental observables.

U2 - 10.1093/protein/gzab002

DO - 10.1093/protein/gzab002

M3 - Review

C2 - 33724431

AN - SCOPUS:85103227627

VL - 34

JO - Protein Engineering, Design and Selection

JF - Protein Engineering, Design and Selection

SN - 1741-0126

M1 - gzab002

ER -

ID: 260744008