In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection
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In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection. / Niola, Francesco; Dagnæs-Hansen, Frederik; Frödin, Morten.
CRISPR Gene Editing: Methods and Protocols. red. / Yonglun Luo. Bind 1961 New York, NY : Humana Press, 2019. s. 329-341 20 (Methods in molecular biology (Clifton, N.J.)).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning
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TY - CHAP
T1 - In Vivo Editing of the Adult Mouse Liver Using CRISPR/Cas9 and Hydrodynamic Tail Vein Injection
AU - Niola, Francesco
AU - Dagnæs-Hansen, Frederik
AU - Frödin, Morten
PY - 2019
Y1 - 2019
N2 - CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.
AB - CRISPR/Cas9 technology allows facile modification of the genome in virtually any desired way through the use of easily designed plasmid constructs that express a gRNA targeting a genomic site-of-interest and Cas9. Hydrodynamic tail vein injection, on the other hand, is a simple method to deliver "naked" plasmid DNA to 5-40% of the hepatocytes of the liver of adult mice. Here, we describe how these two techniques can be combined to create a workflow for fast, easy, and cost-efficient in vivo genome editing of the adult mouse liver. Using this method, large cohorts of mice with genetically modified livers can be established within 3 weeks to generate models for gene function in normal physiology and diseases of the liver.
KW - Animals
KW - CRISPR-Cas Systems/genetics
KW - Gene Editing
KW - Hepatocytes/metabolism
KW - Liver/metabolism
KW - Mice
KW - Plasmids/genetics
KW - RNA, Guide/genetics
U2 - 10.1007/978-1-4939-9170-9_20
DO - 10.1007/978-1-4939-9170-9_20
M3 - Book chapter
C2 - 30912055
SN - 978-1-4939-9169-3
VL - 1961
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 329
EP - 341
BT - CRISPR Gene Editing
A2 - Luo, Yonglun
PB - Humana Press
CY - New York, NY
ER -
ID: 231415213