Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1
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- Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a L150P mutation in PSEN-1
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Induced pluripotent stem cells (iPSCs) were generated from skin fibroblasts isolated from a 58-year old male with a L150P mutation in the presenilin 1 (PSEN-1) gene, which is responsible for the majority of familial cases of Alzheimer's disease (AD). The iPSC swere established by co-electroporation with episomal plasmids containing hOCT4, hSOX2, hL-MYC, hKLF4, hNANOG, hLIN28, and short hairpin RNA against TP53. The iPSCs contained the specific heterozygous mutation c.449C>T, had normal karyotype, expressed the expected pluripotency genes and displayed in vitro differentiation potential to the three germ layers. The iPSCsmay be useful for studying familialAD pathology in vitro.
Originalsprog | Engelsk |
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Tidsskrift | Stem Cell Research |
Vol/bind | 16 |
Udgave nummer | 1 |
Sider (fra-til) | 110-112 |
Antal sider | 3 |
ISSN | 1873-5061 |
DOI | |
Status | Udgivet - jan. 2016 |
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