Energy taxis drives Campylobacter jejuni toward the most favorable conditions for growth
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Energy taxis drives Campylobacter jejuni toward the most favorable conditions for growth. / Vegge, Christina S; Brøndsted, Lone; Li, Yi-Ping; Bang, Dang D.; Ingmer, Hanne.
I: Applied and Environmental Microbiology, Bind 75, Nr. 16, 2009, s. 5308-5314.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Energy taxis drives Campylobacter jejuni toward the most favorable conditions for growth
AU - Vegge, Christina S
AU - Brøndsted, Lone
AU - Li, Yi-Ping
AU - Bang, Dang D.
AU - Ingmer, Hanne
N1 - Keywords: Animals; Bacterial Proteins; Campylobacter jejuni; Carbon; Cell Line, Tumor; Cells, Cultured; Chemotaxis; Chick Embryo; Chickens; Colon; Electron Transport; Epithelial Cells; Gene Deletion; Humans; Intestines; Membrane Proteins; Movement; Sodium Azide
PY - 2009
Y1 - 2009
N2 - Campylobacter jejuni is a serious food-borne bacterial pathogen in the developed world. Poultry is a major reservoir, and C. jejuni appears highly adapted to the gastrointestinal tract of birds. Several factors are important for chicken colonization and virulence, including a taxis mechanism for environmental navigation. To explore the mechanism of chemotaxis in C. jejuni, we constructed mutants with deletions of five putative mcp (methyl-accepting chemotaxis protein) genes (tlp1, tlp2, tlp3, docB, and docC). Surprisingly, the deletions did not affect the chemotactic behavior of the mutants compared to that of the parental strain. However, the tlp1, tlp3, docB, and docC mutant strains displayed a 10-fold decrease in the ability to invade human epithelial and chicken embryo cells, hence demonstrating that the corresponding proteins affect the host interaction. L-Asparagine, formate, D-lactate, and chicken mucus were identified as new attractants of C. jejuni, and we observed that chemical substances promoting tactic attraction are all known to support the growth of this organism. The attractants could be categorized as carbon sources and electron donors and acceptors, and we furthermore observed a correlation between an attractant's potency and its efficiency as an energy source. The tactic attraction was inhibited by the respiratory inhibitors HQNO (2-n-heptyl-4-hydroxyquinoline N-oxide) and sodium azide, which significantly reduce energy production by oxidative phosphorylation. These findings strongly indicate that energy taxis is the primary force in environmental navigation by C. jejuni and that this mechanism drives the organism toward the optimal chemical conditions for energy generation and colonization.
AB - Campylobacter jejuni is a serious food-borne bacterial pathogen in the developed world. Poultry is a major reservoir, and C. jejuni appears highly adapted to the gastrointestinal tract of birds. Several factors are important for chicken colonization and virulence, including a taxis mechanism for environmental navigation. To explore the mechanism of chemotaxis in C. jejuni, we constructed mutants with deletions of five putative mcp (methyl-accepting chemotaxis protein) genes (tlp1, tlp2, tlp3, docB, and docC). Surprisingly, the deletions did not affect the chemotactic behavior of the mutants compared to that of the parental strain. However, the tlp1, tlp3, docB, and docC mutant strains displayed a 10-fold decrease in the ability to invade human epithelial and chicken embryo cells, hence demonstrating that the corresponding proteins affect the host interaction. L-Asparagine, formate, D-lactate, and chicken mucus were identified as new attractants of C. jejuni, and we observed that chemical substances promoting tactic attraction are all known to support the growth of this organism. The attractants could be categorized as carbon sources and electron donors and acceptors, and we furthermore observed a correlation between an attractant's potency and its efficiency as an energy source. The tactic attraction was inhibited by the respiratory inhibitors HQNO (2-n-heptyl-4-hydroxyquinoline N-oxide) and sodium azide, which significantly reduce energy production by oxidative phosphorylation. These findings strongly indicate that energy taxis is the primary force in environmental navigation by C. jejuni and that this mechanism drives the organism toward the optimal chemical conditions for energy generation and colonization.
KW - Former LIFE faculty
KW - Animals
KW - Bacterial Proteins
KW - Campylobacter jejuni
KW - Carbon
KW - Cell Line
KW - Tumor
KW - Cultured
KW - Chemotaxis
KW - Chick Embryo
KW - Chickens
KW - Colon
KW - Electron Transport
KW - Epithelial Cells
KW - Gene Deletion
KW - Humans
KW - Intestines
KW - Membrane Proteins
KW - Movement
KW - Sodium Azide
U2 - 10.1128/AEM.00287-09
DO - 10.1128/AEM.00287-09
M3 - Journal article
C2 - 19542337
VL - 75
SP - 5308
EP - 5314
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 16
ER -
ID: 16129846