Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry

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Standard

Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry. / Mareck, Ute; Guddat, Sven; Schwenke, Anne; Beuck, Simon; Geyer, Hans; Flenker, Ulrich; Elers, Jimmi; Backer, Vibeke; Thevis, Mario; Schänzer, Wilhelm.

I: Drug Testing and Analysis, Bind 3, Nr. 11-12, 2012, s. 820-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Mareck, U, Guddat, S, Schwenke, A, Beuck, S, Geyer, H, Flenker, U, Elers, J, Backer, V, Thevis, M & Schänzer, W 2012, 'Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry', Drug Testing and Analysis, bind 3, nr. 11-12, s. 820-7. https://doi.org/10.1002/dta.367

APA

Mareck, U., Guddat, S., Schwenke, A., Beuck, S., Geyer, H., Flenker, U., Elers, J., Backer, V., Thevis, M., & Schänzer, W. (2012). Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry. Drug Testing and Analysis, 3(11-12), 820-7. https://doi.org/10.1002/dta.367

Vancouver

Mareck U, Guddat S, Schwenke A, Beuck S, Geyer H, Flenker U o.a. Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry. Drug Testing and Analysis. 2012;3(11-12):820-7. https://doi.org/10.1002/dta.367

Author

Mareck, Ute ; Guddat, Sven ; Schwenke, Anne ; Beuck, Simon ; Geyer, Hans ; Flenker, Ulrich ; Elers, Jimmi ; Backer, Vibeke ; Thevis, Mario ; Schänzer, Wilhelm. / Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry. I: Drug Testing and Analysis. 2012 ; Bind 3, Nr. 11-12. s. 820-7.

Bibtex

@article{0fa9122841464944ad437a63e4b81953,

title = "Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry",

abstract = "The determination of salbutamol and its glucuronide in human urine following the inhalative and oral administration of therapeutic doses of salbutamol preparations was performed by means of direct urine injection utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) and employing d(3)-salbutamol and d(3)-salbutamol glucuronide as internal standards. Unconjugated salbutamol was detected in all administration study urine samples. Salbutamol concentrations following inhalation were commonly (99%) below 1000 ng/ml whereas values after oral administration frequently (48%) exceeded this threshold. While salbutamol glucuronide was not detected in urine samples collected after inhalation of the drug, 26 out of 82 specimens obtained after oral application contained salbutamol glucuronide with a peak value of 63 ng/ml. The percentage of salbutamol glucuronide compared to unconjugated salbutamol was less than 3%. Authentic doping control urine samples indicating screening results for salbutamol less than 1000 ng/ml, showed salbutamol glucuronide concentrations between 2 and 6 ng/ml, whereas adverse analytical findings resulting from salbutamol levels higher than 1000 ng/ml, had salbutamol glucuronide values between 8 and 15 ng/ml. The approach enabled the rapid determination of salbutamol and its glucuronic acid conjugate in human urine and represents an alternative to existing procedures since time-consuming hydrolysis or derivatization steps were omitted. Moreover, the excretion of salbutamol glucuronide in human urine following the administration of salbutamol was proven.",

author = "Ute Mareck and Sven Guddat and Anne Schwenke and Simon Beuck and Hans Geyer and Ulrich Flenker and Jimmi Elers and Vibeke Backer and Mario Thevis and Wilhelm Sch{\"a}nzer",

note = "Copyright {\textcopyright} 2011 John Wiley & Sons, Ltd.",

year = "2012",

doi = "10.1002/dta.367",

language = "English",

volume = "3",

pages = "820--7",

journal = "Drug Testing and Analysis",

issn = "1942-7603",

publisher = "JohnWiley & Sons Ltd",

number = "11-12",

}

RIS

TY - JOUR

T1 - Determination of salbutamol and salbutamol glucuronide in human urine by means of liquid chromatography-tandem mass spectrometry

AU - Mareck, Ute

AU - Guddat, Sven

AU - Schwenke, Anne

AU - Beuck, Simon

AU - Geyer, Hans

AU - Flenker, Ulrich

AU - Elers, Jimmi

AU - Backer, Vibeke

AU - Thevis, Mario

AU - Schänzer, Wilhelm

PY - 2012

Y1 - 2012

N2 - The determination of salbutamol and its glucuronide in human urine following the inhalative and oral administration of therapeutic doses of salbutamol preparations was performed by means of direct urine injection utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) and employing d(3)-salbutamol and d(3)-salbutamol glucuronide as internal standards. Unconjugated salbutamol was detected in all administration study urine samples. Salbutamol concentrations following inhalation were commonly (99%) below 1000 ng/ml whereas values after oral administration frequently (48%) exceeded this threshold. While salbutamol glucuronide was not detected in urine samples collected after inhalation of the drug, 26 out of 82 specimens obtained after oral application contained salbutamol glucuronide with a peak value of 63 ng/ml. The percentage of salbutamol glucuronide compared to unconjugated salbutamol was less than 3%. Authentic doping control urine samples indicating screening results for salbutamol less than 1000 ng/ml, showed salbutamol glucuronide concentrations between 2 and 6 ng/ml, whereas adverse analytical findings resulting from salbutamol levels higher than 1000 ng/ml, had salbutamol glucuronide values between 8 and 15 ng/ml. The approach enabled the rapid determination of salbutamol and its glucuronic acid conjugate in human urine and represents an alternative to existing procedures since time-consuming hydrolysis or derivatization steps were omitted. Moreover, the excretion of salbutamol glucuronide in human urine following the administration of salbutamol was proven.

AB - The determination of salbutamol and its glucuronide in human urine following the inhalative and oral administration of therapeutic doses of salbutamol preparations was performed by means of direct urine injection utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) and employing d(3)-salbutamol and d(3)-salbutamol glucuronide as internal standards. Unconjugated salbutamol was detected in all administration study urine samples. Salbutamol concentrations following inhalation were commonly (99%) below 1000 ng/ml whereas values after oral administration frequently (48%) exceeded this threshold. While salbutamol glucuronide was not detected in urine samples collected after inhalation of the drug, 26 out of 82 specimens obtained after oral application contained salbutamol glucuronide with a peak value of 63 ng/ml. The percentage of salbutamol glucuronide compared to unconjugated salbutamol was less than 3%. Authentic doping control urine samples indicating screening results for salbutamol less than 1000 ng/ml, showed salbutamol glucuronide concentrations between 2 and 6 ng/ml, whereas adverse analytical findings resulting from salbutamol levels higher than 1000 ng/ml, had salbutamol glucuronide values between 8 and 15 ng/ml. The approach enabled the rapid determination of salbutamol and its glucuronic acid conjugate in human urine and represents an alternative to existing procedures since time-consuming hydrolysis or derivatization steps were omitted. Moreover, the excretion of salbutamol glucuronide in human urine following the administration of salbutamol was proven.

U2 - 10.1002/dta.367

DO - 10.1002/dta.367

M3 - Journal article

C2 - 22081503

VL - 3

SP - 820

EP - 827

JO - Drug Testing and Analysis

JF - Drug Testing and Analysis

SN - 1942-7603

IS - 11-12

ER -

ID: 40141071

Research