yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs

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yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs. / Frank, Stefan; Ahuja, Gaurav; Bartsch, Deniz; Russ, Nicole; Yao, Wenjie; Kuo, Joseph Chao-Chung; Derks, Jens-Peter; Akhade, Vijay Suresh; Kargapolova, Yulia; Georgomanolis, Theodore; Messling, Jan-Erik; Gramm, Marie; Brant, Lilija; Rehimi, Rizwan; Vargas, Natalia Emilse; Kuroczik, Alina; Yang, Tsun-Po; Sahito, Raja Ghazanfar Ali; Franzen, Julia; Hescheler, Juergen; Sachinidis, Agapios; Peifer, Martin; Rada-Iglesias, Alvaro; Kanduri, Meena; Costa, Ivan G; Kanduri, Chandrasekhar; Papantonis, Argyris; Kurian, Leo.

In: Cell Stem Cell, Vol. 24, No. 2, 07.02.2019, p. 318-327.e8.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Frank, S, Ahuja, G, Bartsch, D, Russ, N, Yao, W, Kuo, JC-C, Derks, J-P, Akhade, VS, Kargapolova, Y, Georgomanolis, T, Messling, J-E, Gramm, M, Brant, L, Rehimi, R, Vargas, NE, Kuroczik, A, Yang, T-P, Sahito, RGA, Franzen, J, Hescheler, J, Sachinidis, A, Peifer, M, Rada-Iglesias, A, Kanduri, M, Costa, IG, Kanduri, C, Papantonis, A & Kurian, L 2019, 'yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs', Cell Stem Cell, vol. 24, no. 2, pp. 318-327.e8. https://doi.org/10.1016/j.stem.2018.11.005

APA

Frank, S., Ahuja, G., Bartsch, D., Russ, N., Yao, W., Kuo, J. C-C., ... Kurian, L. (2019). yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs. Cell Stem Cell, 24(2), 318-327.e8. https://doi.org/10.1016/j.stem.2018.11.005

Vancouver

Frank S, Ahuja G, Bartsch D, Russ N, Yao W, Kuo JC-C et al. yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs. Cell Stem Cell. 2019 Feb 7;24(2):318-327.e8. https://doi.org/10.1016/j.stem.2018.11.005

Author

Frank, Stefan ; Ahuja, Gaurav ; Bartsch, Deniz ; Russ, Nicole ; Yao, Wenjie ; Kuo, Joseph Chao-Chung ; Derks, Jens-Peter ; Akhade, Vijay Suresh ; Kargapolova, Yulia ; Georgomanolis, Theodore ; Messling, Jan-Erik ; Gramm, Marie ; Brant, Lilija ; Rehimi, Rizwan ; Vargas, Natalia Emilse ; Kuroczik, Alina ; Yang, Tsun-Po ; Sahito, Raja Ghazanfar Ali ; Franzen, Julia ; Hescheler, Juergen ; Sachinidis, Agapios ; Peifer, Martin ; Rada-Iglesias, Alvaro ; Kanduri, Meena ; Costa, Ivan G ; Kanduri, Chandrasekhar ; Papantonis, Argyris ; Kurian, Leo. / yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs. In: Cell Stem Cell. 2019 ; Vol. 24, No. 2. pp. 318-327.e8.

Bibtex

@article{6f00b9df2a5d4b40ad499681cc7c3246,
title = "yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs",
abstract = "Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.",
author = "Stefan Frank and Gaurav Ahuja and Deniz Bartsch and Nicole Russ and Wenjie Yao and Kuo, {Joseph Chao-Chung} and Jens-Peter Derks and Akhade, {Vijay Suresh} and Yulia Kargapolova and Theodore Georgomanolis and Jan-Erik Messling and Marie Gramm and Lilija Brant and Rizwan Rehimi and Vargas, {Natalia Emilse} and Alina Kuroczik and Tsun-Po Yang and Sahito, {Raja Ghazanfar Ali} and Julia Franzen and Juergen Hescheler and Agapios Sachinidis and Martin Peifer and Alvaro Rada-Iglesias and Meena Kanduri and Costa, {Ivan G} and Chandrasekhar Kanduri and Argyris Papantonis and Leo Kurian",
note = "Copyright {\circledC} 2018 Elsevier Inc. All rights reserved.",
year = "2019",
month = "2",
day = "7",
doi = "10.1016/j.stem.2018.11.005",
language = "English",
volume = "24",
pages = "318--327.e8",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - yylncT Defines a Class of Divergently Transcribed lncRNAs and Safeguards the T-mediated Mesodermal Commitment of Human PSCs

AU - Frank, Stefan

AU - Ahuja, Gaurav

AU - Bartsch, Deniz

AU - Russ, Nicole

AU - Yao, Wenjie

AU - Kuo, Joseph Chao-Chung

AU - Derks, Jens-Peter

AU - Akhade, Vijay Suresh

AU - Kargapolova, Yulia

AU - Georgomanolis, Theodore

AU - Messling, Jan-Erik

AU - Gramm, Marie

AU - Brant, Lilija

AU - Rehimi, Rizwan

AU - Vargas, Natalia Emilse

AU - Kuroczik, Alina

AU - Yang, Tsun-Po

AU - Sahito, Raja Ghazanfar Ali

AU - Franzen, Julia

AU - Hescheler, Juergen

AU - Sachinidis, Agapios

AU - Peifer, Martin

AU - Rada-Iglesias, Alvaro

AU - Kanduri, Meena

AU - Costa, Ivan G

AU - Kanduri, Chandrasekhar

AU - Papantonis, Argyris

AU - Kurian, Leo

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2019/2/7

Y1 - 2019/2/7

N2 - Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.

AB - Human protein-coding genes are often accompanied by divergently transcribed non-coding RNAs whose functions, especially in cell fate decisions, are poorly understood. Using an hESC-based cardiac differentiation model, we define a class of divergent lncRNAs, termed yin yang lncRNAs (yylncRNAs), that mirror the cell-type-specific expression pattern of their protein-coding counterparts. yylncRNAs are preferentially encoded from the genomic loci of key developmental cell fate regulators. Most yylncRNAs are spliced polyadenylated transcripts showing comparable expression patterns in vivo in mouse and in human embryos. Signifying their developmental function, the key mesoderm specifier BRACHYURY (T) is accompanied by yylncT, which localizes to the active T locus during mesoderm commitment. yylncT binds the de novo DNA methyltransferase DNMT3B, and its transcript is required for activation of the T locus, with yylncT depletion specifically abolishing mesodermal commitment. Collectively, we report a lncRNA-mediated regulatory layer safeguarding embryonic cell fate transitions.

U2 - 10.1016/j.stem.2018.11.005

DO - 10.1016/j.stem.2018.11.005

M3 - Journal article

VL - 24

SP - 318-327.e8

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 2

ER -

ID: 225433404