Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice. / Fjære, Even; Andersen, Charlotte; Myrmel, Lene Secher; Petersen, Rasmus Koefoed; Hansen, Jakob Bondo; Tastesen, Hanne Sørup; Mandrup-Poulsen, Thomas; Brünner, Nils; Kristiansen, Karsten; Madsen, Lise; Rømer, Maria Unni Koefoed.

In: PloS one, Vol. 10, No. 7, e0132910, 2015.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Fjære, E, Andersen, C, Myrmel, LS, Petersen, RK, Hansen, JB, Tastesen, HS, Mandrup-Poulsen, T, Brünner, N, Kristiansen, K, Madsen, L & Rømer, MUK 2015, 'Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice', PloS one, vol. 10, no. 7, e0132910. https://doi.org/10.1371/journal.pone.0132910

APA

Fjære, E., Andersen, C., Myrmel, L. S., Petersen, R. K., Hansen, J. B., Tastesen, H. S., ... Rømer, M. U. K. (2015). Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice. PloS one, 10(7), [e0132910]. https://doi.org/10.1371/journal.pone.0132910

Vancouver

Fjære E, Andersen C, Myrmel LS, Petersen RK, Hansen JB, Tastesen HS et al. Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice. PloS one. 2015;10(7). e0132910. https://doi.org/10.1371/journal.pone.0132910

Author

Fjære, Even ; Andersen, Charlotte ; Myrmel, Lene Secher ; Petersen, Rasmus Koefoed ; Hansen, Jakob Bondo ; Tastesen, Hanne Sørup ; Mandrup-Poulsen, Thomas ; Brünner, Nils ; Kristiansen, Karsten ; Madsen, Lise ; Rømer, Maria Unni Koefoed. / Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice. In: PloS one. 2015 ; Vol. 10, No. 7.

Bibtex

@article{c4f807de38054736ae4041a5f8cde54a,
title = "Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice",
abstract = "BACKGROUND: Plasma levels of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are elevated in obesity and obesity-related disorders, such as steatosis, but the metabolic role of TIMP-1 is unclear. Here we investigated how the presence or absence of TIMP-1 affected the development of diet-induced glucose intolerance and hepatic steatosis using the Timp1 null mice.METHODS: Timp1 knockout (TKO) and wild type (TWT) mice were fed chow, high-fat diet (HFD) or intermediate fat and sucrose diet (IFSD). We determined body weight, body composition, lipid content of the liver, energy intake, energy expenditure, oral glucose tolerance, as well as insulin tolerance. In addition, the histology of liver and adipose tissues was examined and expression of selected genes involved in lipid metabolism and inflammation in liver and adipose tissues was determined by RT-qPCR.RESULTS: TKO mice gained less weight and had lower energy efficiency than TWT mice when fed HFD, but not when fed chow or IFSD. Importantly, TKO mice were protected from development of HFD- as well as IFSD-induced glucose intolerance, hepatic steatosis, and altered expression of genes involved in hepatic lipid metabolism and inflammation.CONCLUSION: Collectively, our results indicate that TIMP-1 contributes to the development of diet-induced hepatic steatosis and glucose intolerance and may be a potential therapeutic target.",
author = "Even Fj{\ae}re and Charlotte Andersen and Myrmel, {Lene Secher} and Petersen, {Rasmus Koefoed} and Hansen, {Jakob Bondo} and Tastesen, {Hanne S{\o}rup} and Thomas Mandrup-Poulsen and Nils Br{\"u}nner and Karsten Kristiansen and Lise Madsen and R{\o}mer, {Maria Unni Koefoed}",
year = "2015",
doi = "10.1371/journal.pone.0132910",
language = "English",
volume = "10",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

RIS

TY - JOUR

T1 - Tissue inhibitor of matrix metalloproteinase-1 is required for high-fat diet-induced glucose intolerance and hepatic steatosis in mice

AU - Fjære, Even

AU - Andersen, Charlotte

AU - Myrmel, Lene Secher

AU - Petersen, Rasmus Koefoed

AU - Hansen, Jakob Bondo

AU - Tastesen, Hanne Sørup

AU - Mandrup-Poulsen, Thomas

AU - Brünner, Nils

AU - Kristiansen, Karsten

AU - Madsen, Lise

AU - Rømer, Maria Unni Koefoed

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Plasma levels of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are elevated in obesity and obesity-related disorders, such as steatosis, but the metabolic role of TIMP-1 is unclear. Here we investigated how the presence or absence of TIMP-1 affected the development of diet-induced glucose intolerance and hepatic steatosis using the Timp1 null mice.METHODS: Timp1 knockout (TKO) and wild type (TWT) mice were fed chow, high-fat diet (HFD) or intermediate fat and sucrose diet (IFSD). We determined body weight, body composition, lipid content of the liver, energy intake, energy expenditure, oral glucose tolerance, as well as insulin tolerance. In addition, the histology of liver and adipose tissues was examined and expression of selected genes involved in lipid metabolism and inflammation in liver and adipose tissues was determined by RT-qPCR.RESULTS: TKO mice gained less weight and had lower energy efficiency than TWT mice when fed HFD, but not when fed chow or IFSD. Importantly, TKO mice were protected from development of HFD- as well as IFSD-induced glucose intolerance, hepatic steatosis, and altered expression of genes involved in hepatic lipid metabolism and inflammation.CONCLUSION: Collectively, our results indicate that TIMP-1 contributes to the development of diet-induced hepatic steatosis and glucose intolerance and may be a potential therapeutic target.

AB - BACKGROUND: Plasma levels of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are elevated in obesity and obesity-related disorders, such as steatosis, but the metabolic role of TIMP-1 is unclear. Here we investigated how the presence or absence of TIMP-1 affected the development of diet-induced glucose intolerance and hepatic steatosis using the Timp1 null mice.METHODS: Timp1 knockout (TKO) and wild type (TWT) mice were fed chow, high-fat diet (HFD) or intermediate fat and sucrose diet (IFSD). We determined body weight, body composition, lipid content of the liver, energy intake, energy expenditure, oral glucose tolerance, as well as insulin tolerance. In addition, the histology of liver and adipose tissues was examined and expression of selected genes involved in lipid metabolism and inflammation in liver and adipose tissues was determined by RT-qPCR.RESULTS: TKO mice gained less weight and had lower energy efficiency than TWT mice when fed HFD, but not when fed chow or IFSD. Importantly, TKO mice were protected from development of HFD- as well as IFSD-induced glucose intolerance, hepatic steatosis, and altered expression of genes involved in hepatic lipid metabolism and inflammation.CONCLUSION: Collectively, our results indicate that TIMP-1 contributes to the development of diet-induced hepatic steatosis and glucose intolerance and may be a potential therapeutic target.

U2 - 10.1371/journal.pone.0132910

DO - 10.1371/journal.pone.0132910

M3 - Journal article

VL - 10

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

M1 - e0132910

ER -

ID: 145522923