The ubiquitin ligase HectH9 regulates transcriptional activation by Myc and is essential for tumor cell proliferation.

Research output: Contribution to journalJournal articleResearchpeer-review

Sovana Adhikary, Federica Marinoni, Andreas Hock, Esther Hulleman, Nikita Popov, Rudi Beier, Sandra Bernard, Micaela Quarto, Maria Capra, Stephan Goettig, Ulrike Kogel, Martin Scheffner, Kristian Helin, Martin Eilers

The Myc oncoprotein forms a binary activating complex with its partner protein, Max, and a ternary repressive complex that, in addition to Max, contains the zinc finger protein Miz1. Here we show that the E3 ubiquitin ligase HectH9 ubiquitinates Myc in vivo and in vitro, forming a lysine 63-linked polyubiquitin chain. Miz1 inhibits this ubiquitination. HectH9-mediated ubiquitination of Myc is required for transactivation of multiple target genes, recruitment of the coactivator p300, and induction of cell proliferation by Myc. HectH9 is overexpressed in multiple human tumors and is essential for proliferation of a subset of tumor cells. Our results suggest that site-specific ubiquitination regulates the switch between an activating and a repressive state of the Myc protein, and they suggest a strategy to interfere with Myc function in vivo.
Original languageEnglish
JournalCell
Volume123
Issue number3
Pages (from-to)409-21
Number of pages12
ISSN0092-8674
DOIs
Publication statusPublished - 2005

Bibliographical note

Keywords: Amino Acid Sequence; Animals; Cell Line, Tumor; Cell Proliferation; DNA-Binding Proteins; Genes, myc; Humans; Kruppel-Like Transcription Factors; Mice; Molecular Sequence Data; Neoplasms; Polyubiquitin; Proto-Oncogene Proteins c-myc; Trans-Activation (Genetics); Transcription Factors; Ubiquitin-Protein Ligases; p300-CBP Transcription Factors

ID: 5053498