The Impact of Preprocessing Pipeline Choice in Univariate and Multivariate Analyses of PET Data

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Martin Nørgaard, Douglas N. Greve, Claus Svarer, Stephen C. Strother, Gitte M. Knudsen, Melanie Ganz

It has long been recognized that the data preprocessing chain is a critical part of a neuroimaging experiment. In this work we evaluate the impact of preprocessing choices in univariate and multivariate analyses of Positron Emission Tomography (PET) data. Thirty healthy participants were scanned twice in a High-Resolution Research Tomography PET scanner with the serotonin transporter (5-HTT) radioligand [11 C]DASB. Binding potentials (BPND) from 14 brain regions are quantified with 384 different preprocessing choices. A univariate paired t-Test is applied to each region and for each preprocessing choice, and corrected for multiple comparisons using FDR within each pipeline. Additionally, a multivariate Linear Discriminant Analysis (LDA) model is used to discriminate test and retest BPND, and the model performance is evaluated using a repeated cross-validation framework with permutations. The univariate analysis revealed several significant differences in 5-HTT BPND across brain regions, depending on the preprocessing choice. The classification accuracy of the multivariate LDA model varied from 37% to 70% depending on the choice of preprocessing, and could reasonably be modeled with a normal distribution centered at 51% accuracy. In spite of correcting for multiple comparisons, the univariate model with varying preprocessing choices is more likely to generate false-positive results compared to a simple multivariate analysis model evaluated with cross-validation and permutations.

Original languageEnglish
Title of host publication2018 International Workshop on Pattern Recognition in Neuroimaging, PRNI 2018
Number of pages4
PublisherIEEE
Publication date2018
Article number8423962
ISBN (Print)9781538668597
DOIs
Publication statusPublished - 2018
Event2018 International Workshop on Pattern Recognition in Neuroimaging, PRNI 2018 - Singapore, Singapore
Duration: 12 Jun 201814 Jun 2018

Conference

Conference2018 International Workshop on Pattern Recognition in Neuroimaging, PRNI 2018
LandSingapore
BySingapore
Periode12/06/201814/06/2018

ID: 203671902