The effect of smoking on the urinary excretion of 8-oxodG and 8-oxoGuo in patients with type 2 diabetes

Research output: Contribution to journalJournal articleResearchpeer-review

Anne-Sofie Sørensen, Laura Kofoed Kjær, Kasper Meidahl Petersen, Trine Henriksen, Vanja Cejvanovic, Oluf Pedersen, Torben Hansen, Cramer Kjeldahl Christensen, Ivan Brandslund, Henrik Enghusen Poulsen

Over the past decades, attention has been paid to understanding the impact of oxidative stress and related modifications of DNA and RNA on various human health risks. A recent meta-analysis comprising 1915 smokers and 3462 non-smokers found a significantly higher level of DNA oxidation measured as urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) excretion in smokers compared with non-smokers in a healthy population. We aimed to investigate if an increased urinary excretion of 8-oxodG in smokers versus never smokers and former smokers could be verified in a population with type 2 diabetes. Additionally, we measured RNA oxidation levels through urinary excretion of 8-oxo-7, 8-dihydroguanosine (8-oxoGuo). Our study included urinary samples from 2721 type 2 diabetic patients, analyzed using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Logistic regression was used to examine the relationship between daily smokers (n = 462) versus former (n = 1341) and never smokers (n = 918) regarding the RNA and DNA oxidation, respectively. We did not find any significant effect of smoking on urinary excretion of 8-oxodG or 8-oxoGuo in our study. Due to a sparse study area, it is still too early to draw any conclusions on smoking and RNA-oxidation. Regarding DNA oxidation, our study suggests that the effect of smoking seen in healthy populations might be attenuated in patients with type 2 diabetes.

Original languageEnglish
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume77
Issue number4
Pages (from-to)253-258
Number of pages6
ISSN0036-5513
DOIs
Publication statusPublished - 2017

    Research areas

  • Journal Article

ID: 174398438