Role of luminal nutrients and endogenous GLP-2 in intestinal adaptation to mid-small bowel resection

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Elizabeth M Dahly, Melanie B Gillingham, Ziwen Guo, Sangita G Murali, David W Nelson, Jens Juul Holst, Denise M Ney

To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.

Original languageEnglish
JournalAmerican Journal of Physiology: Gastrointestinal and Liver Physiology
Volume284
Issue number4
Pages (from-to)G670-82
ISSN0193-1857
DOIs
Publication statusPublished - Apr 2003

    Research areas

  • Adaptation, Physiological, Animals, Apoptosis, Body Weight, Cell Division, Eating, Enterocytes, Glucagon, Glucagon-Like Peptide 2, Glucagon-Like Peptides, Ileum, Jejunum, Male, Parenteral Nutrition, Peptides, Proglucagon, Protein Precursors, Rats, Rats, Sprague-Dawley, Sucrase

ID: 132056367