Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction. / Follin, Bjarke; Ghotbi, Adam Ali; Clemmensen, Andreas Ettrup; Bentsen, Simon; Juhl, Morten; Søndergaard, Rebekka Harary; Lund, Lisbeth Drozd; Haack-Sørensen, Mandana; Hasbak, Philip; Cohen, Smadar; Ripa, Rasmus Sejersten; Kastrup, Jens; Ekblond, Annette; Kjær, Andreas.

In: Stem Cells International, Vol. 2018, 7821461, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Follin, B, Ghotbi, AA, Clemmensen, AE, Bentsen, S, Juhl, M, Søndergaard, RH, Lund, LD, Haack-Sørensen, M, Hasbak, P, Cohen, S, Ripa, RS, Kastrup, J, Ekblond, A & Kjær, A 2018, 'Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction', Stem Cells International, vol. 2018, 7821461. https://doi.org/10.1155/2018/7821461

APA

Follin, B., Ghotbi, A. A., Clemmensen, A. E., Bentsen, S., Juhl, M., Søndergaard, R. H., ... Kjær, A. (2018). Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction. Stem Cells International, 2018, [7821461]. https://doi.org/10.1155/2018/7821461

Vancouver

Follin B, Ghotbi AA, Clemmensen AE, Bentsen S, Juhl M, Søndergaard RH et al. Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction. Stem Cells International. 2018;2018. 7821461. https://doi.org/10.1155/2018/7821461

Author

Follin, Bjarke ; Ghotbi, Adam Ali ; Clemmensen, Andreas Ettrup ; Bentsen, Simon ; Juhl, Morten ; Søndergaard, Rebekka Harary ; Lund, Lisbeth Drozd ; Haack-Sørensen, Mandana ; Hasbak, Philip ; Cohen, Smadar ; Ripa, Rasmus Sejersten ; Kastrup, Jens ; Ekblond, Annette ; Kjær, Andreas. / Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction. In: Stem Cells International. 2018 ; Vol. 2018.

Bibtex

@article{81fd9c6ae8554f3c83419532441b05d1,
title = "Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction",
abstract = "Background: Cell therapy for heart disease has been proven safe and efficacious, despite poor cell retention in the injected area. Improving cell retention is hypothesized to increase the treatment effect. In the present study, human adipose-derived stromal cells (ASCs) were delivered in an in situ forming alginate hydrogel following acute myocardial infarction (AMI) in rats.Methods: ASCs were transduced with luciferase and tested for ASC phenotype. AMI was inducted in nude rats, with subsequent injection of saline (controls), 1 × 106 ASCs in saline or 1 × 106 ASCs in 1{\%} (w/v) alginate hydrogel. ASCs were tracked by bioluminescence and functional measurements were assessed by magnetic resonance imaging (MRI) and 82rubidium positron emission tomography (PET).Results: ASCs in both saline and alginate hydrogel significantly increased the ejection fraction (7.2{\%} and 7.8{\%} at 14 days and 7.2{\%} and 8.0{\%} at 28 days, resp.). After 28 days, there was a tendency for decreased infarct area and increased perfusion, compared to controls. No significant differences were observed between ASCs in saline or alginate hydrogel, in terms of retention and functional salvage.Conclusion: ASCs improved the myocardial function after AMI, but administration in the alginate hydrogel did not further improve retention of the cells or myocardial function.",
author = "Bjarke Follin and Ghotbi, {Adam Ali} and Clemmensen, {Andreas Ettrup} and Simon Bentsen and Morten Juhl and S{\o}ndergaard, {Rebekka Harary} and Lund, {Lisbeth Drozd} and Mandana Haack-S{\o}rensen and Philip Hasbak and Smadar Cohen and Ripa, {Rasmus Sejersten} and Jens Kastrup and Annette Ekblond and Andreas Kj{\ae}r",
year = "2018",
doi = "10.1155/2018/7821461",
language = "English",
volume = "2018",
journal = "Stem Cells International",
issn = "1687-966X",
publisher = "Hindawi Publishing Corporation",

}

RIS

TY - JOUR

T1 - Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction

AU - Follin, Bjarke

AU - Ghotbi, Adam Ali

AU - Clemmensen, Andreas Ettrup

AU - Bentsen, Simon

AU - Juhl, Morten

AU - Søndergaard, Rebekka Harary

AU - Lund, Lisbeth Drozd

AU - Haack-Sørensen, Mandana

AU - Hasbak, Philip

AU - Cohen, Smadar

AU - Ripa, Rasmus Sejersten

AU - Kastrup, Jens

AU - Ekblond, Annette

AU - Kjær, Andreas

PY - 2018

Y1 - 2018

N2 - Background: Cell therapy for heart disease has been proven safe and efficacious, despite poor cell retention in the injected area. Improving cell retention is hypothesized to increase the treatment effect. In the present study, human adipose-derived stromal cells (ASCs) were delivered in an in situ forming alginate hydrogel following acute myocardial infarction (AMI) in rats.Methods: ASCs were transduced with luciferase and tested for ASC phenotype. AMI was inducted in nude rats, with subsequent injection of saline (controls), 1 × 106 ASCs in saline or 1 × 106 ASCs in 1% (w/v) alginate hydrogel. ASCs were tracked by bioluminescence and functional measurements were assessed by magnetic resonance imaging (MRI) and 82rubidium positron emission tomography (PET).Results: ASCs in both saline and alginate hydrogel significantly increased the ejection fraction (7.2% and 7.8% at 14 days and 7.2% and 8.0% at 28 days, resp.). After 28 days, there was a tendency for decreased infarct area and increased perfusion, compared to controls. No significant differences were observed between ASCs in saline or alginate hydrogel, in terms of retention and functional salvage.Conclusion: ASCs improved the myocardial function after AMI, but administration in the alginate hydrogel did not further improve retention of the cells or myocardial function.

AB - Background: Cell therapy for heart disease has been proven safe and efficacious, despite poor cell retention in the injected area. Improving cell retention is hypothesized to increase the treatment effect. In the present study, human adipose-derived stromal cells (ASCs) were delivered in an in situ forming alginate hydrogel following acute myocardial infarction (AMI) in rats.Methods: ASCs were transduced with luciferase and tested for ASC phenotype. AMI was inducted in nude rats, with subsequent injection of saline (controls), 1 × 106 ASCs in saline or 1 × 106 ASCs in 1% (w/v) alginate hydrogel. ASCs were tracked by bioluminescence and functional measurements were assessed by magnetic resonance imaging (MRI) and 82rubidium positron emission tomography (PET).Results: ASCs in both saline and alginate hydrogel significantly increased the ejection fraction (7.2% and 7.8% at 14 days and 7.2% and 8.0% at 28 days, resp.). After 28 days, there was a tendency for decreased infarct area and increased perfusion, compared to controls. No significant differences were observed between ASCs in saline or alginate hydrogel, in terms of retention and functional salvage.Conclusion: ASCs improved the myocardial function after AMI, but administration in the alginate hydrogel did not further improve retention of the cells or myocardial function.

U2 - 10.1155/2018/7821461

DO - 10.1155/2018/7821461

M3 - Journal article

VL - 2018

JO - Stem Cells International

JF - Stem Cells International

SN - 1687-966X

M1 - 7821461

ER -

ID: 200500655