Remote-loading of liposomes with manganese-52 and in vivo evaluation of the stabilities of 52Mn-DOTA and 64Cu-DOTA using radiolabelled liposomes and PET imaging

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Andreas I Jensen, Gregory W Severin, Anders E Hansen, Frederikke P Fliedner, Rasmus Eliasen, Ladan Parhamifar, Andreas Kjær, Thomas L Andresen, Jonas R Henriksen

Liposomes are nanoparticles used in drug delivery that distribute over several days in humans and larger animals. Radiolabeling with long-lived positron emission tomography (PET) radionuclides, such as manganese-52 (52Mn, T½=5.6days), allow the imaging of this biodistribution. We report optimized protocols for radiolabeling liposomes with 52Mn, through both remote-loading and surface labeling. For comparison, liposomes were also remote-loaded and surface labeled with copper-64 (64Cu, T½=12.7h) through conventional means. The chelator DOTA was used in all cases. The in vivo stability of radiometal chelates is widely debated but studies that mimic a realistic in vivo setting are lacking. Therefore, we employed these four radiolabeled liposome types as platforms to demonstrate a new concept for such in vivo evaluation, here of the chelates 52Mn-DOTA and 64Cu-DOTA. This was done by comparing "shielded" remote-loaded with "exposed" surface labeled variants in a CT26 tumor-bearing mouse model. Remote loading (90min at 55°C) and surface labeling (55°C for 2h) of 52Mn gave excellent radiolabeling efficiencies of 97-100% and 98-100% respectively, and the liposome biodistribution was imaged by PET for up to 8days. Liposomes with surface-conjugated 52Mn-DOTA exhibited a significantly shorter plasma half-life (T½=14.4h) when compared to the remote-loaded counterpart (T½=21.3h), whereas surface-conjugated 64Cu-DOTA cleared only slightly faster and non-significantly, when compared to remote-loaded (17.2±2.9h versus 20.3±1.2h). From our data, we conclude the successful remote-loading of liposomes with 52Mn, and furthermore that 52Mn-DOTA may be unstable in vivo whereas 64Cu-DOTA appears suitable for quantitative imaging.

Original languageEnglish
JournalJournal of Controlled Release
Volume269
Pages (from-to)100-109
ISSN0168-3659
DOIs
Publication statusPublished - 2018

    Research areas

  • Animals, Cell Line, Tumor, Chelating Agents/administration & dosage, Copper Radioisotopes/administration & dosage, Heterocyclic Compounds, 1-Ring/administration & dosage, Liposomes, Manganese/administration & dosage, Mice, Inbred BALB C, Neoplasms/diagnostic imaging, Positron-Emission Tomography, Radioisotopes/administration & dosage, Radiopharmaceuticals/administration & dosage, Tissue Distribution

ID: 215369726