Release of PYY from pig intestinal mucosa; luminal and neural regulation

Research output: Contribution to journalJournal articleResearchpeer-review

S P Sheikh, J J Holst, C Orskov, R Ekman, T W Schwartz

The localization, molecular nature and secretion of Peptide YY (PYY), a putative gut hormone belonging to the Pancreatic Polypeptide family of peptides, was studied in pigs. Immunoreactive PYY was identified in a population of endocrine cells in the mucosal epithelium of the pig ileum. Release of PYY was observed in isolated perfused pig ileum in response to luminal stimulation with glucose and vascular administration of the neuropeptide gastrin-releasing peptide (GRP). Electrical stimulation of the vagus nerve supply to the distal small intestine in intact anaesthetized pigs resulted in release of PYY into the circulation. Stimulation of the splanchnic nerves did not affect the basal release of PYY. PYY-immunoreactivity extracted from ileal tissue or released to plasma or perfusate from the ileum was indistinguishable from synthetic porcine PYY by gel filtration and reverse phase HPLC. It is concluded that the secretion of PYY in the pig ileum may be regulated not only by nutritional luminal factors, but also by postsynaptic parasympathetic nerves.

Original languageEnglish
JournalRegulatory Peptides
Issue number3
Pages (from-to)253-66
Number of pages14
Publication statusPublished - Dec 1989

    Research areas

  • Animals, Chromatography, Gel, Chromatography, High Pressure Liquid, Electric Stimulation, Gastrin-Releasing Peptide, Glucose/pharmacology, Ileum/drug effects, Intestinal Mucosa/drug effects, Models, Biological, Peptide YY, Peptides/analysis, Perfusion, Splanchnic Nerves/physiology, Swine, Vagus Nerve/physiology

ID: 194815863